Gauri R Nair

Scientia Advisors LLC

Aug 2011 to 2000
Business Analyst
Cell Biology and Molecular Genetics Department
College Park, MD
Oct 2006 to Jul 2011
Postdoctoral Fellow
Department of Biology
Philadelphia, PA
Sep 2000 to Sep 2006
Graduate Student Researcher
Department of Biology
Philadelphia, PA
Sep 2000 to Sep 2006
Teaching Assistant
Department of Biology
Philadelphia, PA
Aug 2001 to May 2005
Graduate Associate

University of Pennsylvania
Sep 2006
Doctor of Philosophy in Biology
University of Pune
Jun 1993
Master of Science in Biotechnology

Biochemistry: Western Blot analysis, Kd determination, filter binding assays, primer extension, gel-shift, protein engineering and expression in bacteria, purification of tagged and untagged proteins (manual and FPLC), FRET, experienced radioisotope user Immunology: Western Blot analysis, co-immunoprecipitation, ELISA Molecular Biology: RT-PCR, PCR, primer design and selection, DNA/RNA extraction, site-directed mutagenesis, library construction, transformation, restriction digestion, cloning, mini/midi preps, RNA sequencing, Gel electrophoresis, virulence assays, reporter gene assays, Lentivirus replication Cell Biology: Maintenance and growth of primary cell lines, maintenance and growth of bacterial cultures and explants of plant models of crown gall disease Software skills: Adobe Photoshop, Illustrator and ImageReady, Canvas, Microsoft Office, Endnote MacVector, Advanced MS Excel and Powerpoint skills, Graphpad Prism, SigmaPlot, phosphorimager analysis softwares (e.g., ImageQuant)