Inventors:
Biswajit Biswas - Germantown MD, US
Francisco Jesus Malagon Bautista - Germantown MD, US
Luis Angel Estrella - Silver Spring MD, US
Assignee:
United States of America as represented by the Secretary of the Navy - Silver Spring MD
International Classification:
C12Q 1/14
C12N 7/00
C12Q 1/18
C12Q 1/6806
C07K 14/005
C12Q 1/70
C12Q 1/689
Abstract:
Methods of detecting actively growing host organisms, including bacterial organisms, involving the detection of ribonucleic acid (RNA) products produced by unmodified infectious bioagents that infect the host organisms are provided. Related methods of assessing drug susceptibility and resistance are further provided. In addition to these surrogate detection methods, related reaction mixtures, kits, systems, and microfluidic cards are also provided.