Yanju Wang

20130244257, Sep 19, 2013

Mar 2, 2013

13/783283

THE REGENTS OF THE UNIVERSITY OF THE REGENTS CALIFORNIA - , US
Arion F. Hadjioannou (Chatziioannou) - Los Angeles CA, US
Cong Fang - Newbury Park CA, US
Yanju Wang - Los Angeles CA, US
Nam T. Vu - Redondo Beach CA, US

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

G01N 21/75

435 772, 4352872

An immunocapture-based in vitro kinase assay on an integrated polydimethylsiloxane (PDMS) microfluidics platform that can reproducibly measure kinase activity from as few as 3,000 cells is described. For this platform, the standard radiometric P-ATP labeled phosphate transfer assay was adopted. Implementation on a microfluidic device required the development of methods for repeated trapping and mixing of solid-phase affinity micro beads. A solid state beta-particle camera imbedded directly below the microfluidic device was used to provide real-time quantitative detection of the signal from this and other microfluidic radio bioassays. The integrated device can measure ABL protein kinase activity from BCR-ABL positive leukemia patient samples, and can measure the small molecule phosphorylation such as phosphorylation of the deoxycytidine analog F-FAC by deoxycytidine kinase (dCK) isolated from cell lysates.

20120309648, Dec 6, 2012

Jul 7, 2008

12/667861

Jinyi Wang - Los Angeles CA, US
Guodong Sui - Los Angeles CA, US
Kym F. Faull - Los Angeles CA, US
Yanju Wang - Los Angeles CA, US
Wei-Yu Lin - Los Angeles CA, US

The Regents of the University of Calfornia - Oakland CA

C40B 60/12
C40B 30/08

506 11, 506 39

A microfluidic system has a microfluidic mixer and a sample storage component that is in fluid connection with the microfluidic mixer. The microfluidic mixer has a mixing section; a target molecule input section that is in fluid connection with the mixing section, the target molecule input section being suitable to provide a fluid into the mixing section that contains molecules to be targeted by chemical reactions; a first reagent input section that is in fluid connection with the mixing section, the first reagent input section being structured to selectively provide a first reagent selected from a plurality of reagents to test a chemical reaction with the target molecules; a second reagent input section that is in fluid connection with the mixing section, the second reagent input section being structured to selectively provide a second reagent selected from a plurality of reagents to test a chemical reaction with the target molecules and said first reagent; and a neutral fluid input section that is in selectable fluid connection with the sample storage component, the neutral fluid input section being structured to selectively provide a neutral fluid into the sample storage component between successive samples provided to the sample storage component to separate successive samples in a stratified arrangement.