Inventors:
Eugene Tokhtuev - Duluth MN, US
Anatoly Skirda - Duluth MN, US
Viktor Slobodyan - Duluth MN, US
Christopher Owen - Duluth MN, US
Assignee:
Apprise Technologies, Inc. - Duluth MN
International Classification:
G01N 21/00
G01N 21/75
G01N 21/62
G01N 21/76
B32B 5/02
US Classification:
422 8202, 422 50, 422 52, 422 55, 422 56, 422 57, 422 58, 422 681, 422 8205, 422 8206, 422 8207, 422 8209, 436 43, 436164, 436171, 436172
Abstract:
A multichannel fluorosensor includes an optical module and an electronic module combined in a watertight housing with an underwater connector. The fluorosensor has an integral calibrator for periodical sensitivity validation of the fluorosensor. The optical module has one or several excitation channels and one or several emission channels that use a mutual focusing system. To increase efficiency, the excitation and emission channels each have a micro-collimator made with one or more ball lenses. Each excitation channel has a light emitting diode and an optical filter. Each emission channel has a photodiode with a preamplifier and an optical filter. The electronic module connects directly to the optical module and includes a lock-in amplifier, a power supply and a controller with an A/D converter and a connector. The calibrator provides a response proportional to the excitation intensity, and matches with spectral parameter of fluorescence for the analyzed fluorescent substance.