Inventors:
Paul Richard Gotti - Albuquerque NM
Trenton Todd Rees - Albuquerque NM
Assignee:
Syncor International Corporation - Woodland Hills CA
International Classification:
G01N 118
G01N 128
Abstract:
Disclosed is a method for separating leukocytes from platelets. Blood serum is added to a centrifuge tube. A suspension containing leukocytes and platelets is then added to form a layer on top of the blood serum, the layer having a density less than the density of the blood serum. The cells and the layers are centrifuged at a force and for a time sufficient to separate the leukocytes from the platelets. The resulting, isolated leukocytes are then recovered from the centrifuge tube. In some embodiments, a suspension of the leukocyte cells are pretreated by incubating with a stannous ion reducing agent, the suspension having a stannous ion concentration of from about 50. mu. g to about 1000. mu. g stannous ion per 10. sup. 8 leukocyte cells.