Inventors:
Kiyoshi Mizuuchi - Bethesda MD
Michiyo Mizuuchi - Bethesda MD
Toshiro Adachi - Bethesda MD
Assignee:
The United States of America as represented by the Department of Health
and Human Services - Washington DC
International Classification:
C12Q 170
C12Q 168
C12N 1500
Abstract:
The present invention discloses a rapid method of sequencing a relatively large segment of deoxyribonucleic acid. The method in part comprises high frequency insertion of a suitable transposon into a segment of DNA of interest. Preferable use of Mu transposons is described. A plasmid having mini-Mu transposons has been prepared and disclosed.