Inventors:
William S. Adney - Golden CO, US
Todd B. Vinzant - Golden CO, US
Michael E. Himmel - Littleton CO, US
Assignee:
Midwest Research Institute - Kansas City MO
International Classification:
C12N 9/00
C12N 9/44
C11D 3/00
C11D 7/42
C07H 21/04
C12P 21/06
US Classification:
435210, 435 4, 435 6, 435 691, 435183, 435200, 4352523, 4353201, 536 232, 536 237, 510114, 510392, 510515
Abstract:
The invention provides a thermal tolerant mannanase that is a member of the glycoside hydrolase family. The invention further discloses this mannanase as ManA. ManA has been isolated and characterized from. The invention further provides recombinant forms of the identified ManA. Methods of making ManA polypeptides, including fusions, variants, and derivatives, are also disclosed. Methods of using mannanase A, including for the processing of food and for use in food stuffs as bulking agents and the like, are also disclosed.