Inventors:
James J. Collins - Newton MA, US
Tara L. Deans - Somerville MA, US
International Classification:
A01K 67/00
C12N 15/74
C12N 5/071
A61K 45/00
G01N 33/53
A61P 43/00
US Classification:
800 13, 4353201, 435325, 435366, 424 9321, 435 721
Abstract:
The present invention relates generally the field of genetics, in particular methods, compositions and systems for controlling the inducible expression of transgenes, while eliminating background expression of transgene expression. The present invention relates to methods of use of the compositions and systems as disclosed herein for controlling the inducible expression of transgenes while eliminating background expression of transgene expression, such as use in, for example, the in generation of transgenic animals, use in therapeutic application and use in assays. In some embodiments, the present invention relates to a system of controlled expression of RNAi molecules which target binding sites in the untranslated regions of transgene, thereby the expression of the transgene is modulated and leakiness is reduced. The compositions and methods of the present invention can be used to for therapy, prophylaxis, research and diagnostics in diseases and disorders which afflict mammalian species, generation of transgenic animals, in the study of biological processes as well as for enhance performance of agricultural crops.