US Patent:
20070238143, Oct 11, 2007
Inventors:
Wensheng Xia - Santa Fe NM, US
Frauke Rininsland - Santa Fe NM, US
Sriram Kumaraswamy - Santa Fe NM, US
Stuart Kushon - Santa Fe NM, US
Liangde Lu - Albuquerque NM, US
Xiaobo Shi - Los Alamos NM, US
Casey Stankewicz - Santa Fe NM, US
Shannon Wittenburg - Santa Fe NM, US
Komandoor Achyuthan - Santa Fe NM, US
Duncan McBranch - Santa Fe NM, US
David Whitten - Albuquerque NM, US
International Classification:
C12Q 1/48
C12Q 1/42
C07K 14/47
US Classification:
435015000, 435021000, 530409000
Abstract:
Reagents and assays for kinase, phosphatase and protease enzyme activity which employ metal ion-phosphate ligand specific binding and fluorescent polymer superquenching are described. The assays provide a general platform for the measurement of kinase, phosphatase and protease enzyme activity using peptide and protein substrates. Reagents and assays based on DNA hybridization and reagents and assays for proteins which employ aptamers, antibodies and other ligands are also described.