Inventors:
Yi Li - Miami FL
Carole Young - Miami FL
Timothy J. Fischer - Raleigh NC
James H. Carter - Plantation FL
Sergio C. Veulens - Miami FL
Assignee:
Coulter International Corp. - Miami FL
International Classification:
G01N 3348
G01N 3100
Abstract:
A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enable the differentiation of at least two subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system find use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an ethoxylated long chain compound based lysis reagent with an acid and a solubilizer, and a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples, as well as other fluid samples such as bone marrow.