Sam Tennison

Fairfax County Public Schools

Feb 2009 to 2000
Substitute Teacher
Fairfax County Public Schools

Mar 2010 to Sep 2011
Core Technician
William and Mary Biology Department
Williamsburg, VA
Jul 2008 to Dec 2008
Tech Assistant
College of William and Mary
Williamsburg, VA
Nov 2007 to Dec 2008
Undergraduate Researcher
William and Mary Dance Department
Williamsburg, VA
Sep 2007 to Dec 2008
Cashier
William and Mary Dance Department
Williamsburg, VA
Apr 2007 to Dec 2008
Piano Accompanist
Garden
Williamsburg, VA
Mar 2007 to May 2007
Sales Associate
EDP

Jun 2006 to Aug 2006
Cook Helper
Store 4601
Fairfax, VA
Nov 2004 to Jul 2005
Sales Specialist (Flooring)

George Washington University
Washington, DC
Jan 2010 to Jan 2012
MS in Molecular Biology
University of California - Berkley
Jan 2010 to Jul 2010
BS in Biochemistry & Statistics
College of William and Mary
Williamsburg, VA
Aug 2005 to Jan 2009

Laboratory Skills & Techniques . Basic set-up for PCR (Polymerase Chain Reaction) using ExTaq, GoTaq, and Taq Gold protocols . Basic set-up for 3-prime RACE (Rapid Amplification of c-DNA Ends) PCR protocol by Ambion . Design and set-up of degenerate PCR to isolate suspect gene orthologues using phylogenetic analysis of known genes in extant plant lineages (Arabidopsis, Ephedra, Selaginella) . Genomic DNA Extraction from Plant (Arabidopsis, Ephedra, Selaginella) tissues using organic method (1:8 chloroform:octanol) . Total RNA Extraction from Plant (Arabidopsis, Ephedra, Selaginella) tissues using RNeasy kit (Qiagen) . Clone plasmid vectors into chemically competent E. coli (TOP10 E. coli) . Set an agar plate with kanamycin/ampicillin for E. coli medium, for blue-white selection of transformed E. coli colonies . Extract plasmid vector from E. coli using Qiagen Miniprep microcentrifuge protocol . Set-up and run gel electrophoresis with agarose gel (0.7% - 2.0%) . Stain agarose gel with ethydium bromide or SYBR-Safe DNA staining solution . Conduct Southern blot with chemoluminscent-labeled probes according to manufacturer protocol . Extraction of DNA (nuclear and mtDNA) in a forensic laboratory using Qiagan QIAamp procedure, 5% Chelex, EZ1 robots, and BioSprint robots . Preparation and set-up for PCR targeting nuclear genomic sequences in humans, plants (Arabidopsis, Ephedra, Selaginella); mtDNA sequences in humans. - Set-up for ABI Identifiler, Y-filer - Set-up Human mtDNA HVR-I sequence (GWU) . Preparation of polyacrylamide gel plates for DNA gel electrophoresis STR analysis . Basic handling of Hitachi FMBIO polyacrylamide scanner for DNA gel electrophoresis STR analysis . Basic handling of STR analysis software - GeneMapper (ABI), and GeneMarker . Basic handling of DNA sequence software - Sequencher . Basic handling of sensitive biological and organic substrates - research and forensic environments . Basic handling of autoclave operation . Proper safety and handling of liquid nitrogen