Inventors:
Ramkumar Mandalam - Union City CA, US
Saadia Faouzi - Daly City CA, US
Isabelle Nadeau - San Francisco CA, US
Namitha Rao - San Jose CA, US
Melissa K. Carpenter - London, CA
Lakshmi Rambhatla - Redwood City CA, US
Assignee:
Geron Corporation - Menlo Park CA
International Classification:
C12N 5/00
C12N 5/10
C12N 5/071
A61K 49/00
US Classification:
435377, 435366, 435370, 435363, 435325, 424 91
Abstract:
This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
