Inventors:
Samuel Nochumson - The Woodlands TX, US
Ross Durland - The Woodlands TX, US
Audrey Yu-Speight - The Woodlands TX, US
John Welp - Willis TX, US
Rexford Hayes - Houston TX, US
Assignee:
Valentis, Inc. - Burlingame CA
International Classification:
C07H 1/06
C12P 19/34
US Classification:
536 254, 536 253, 536 2531, 536 231
Abstract:
A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinant cell. Effective, contgrollable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and nuetralization.