Robert David Juncosa

6485690, Nov 26, 2002

May 27, 1999

09/321410

Dale R. Pfost - Pennington NJ
Sheila H. DeWitt - Stockton NJ
Chao Lin - Monmouth Junction NJ
Rolf E. Swenson - Princeton NJ
Robert D. Juncosa - Yardley PA
Gary J. Schnerr - Pennington NJ
Sheldon M. Kugelmass - New York NY

Orchid BioSciences, Inc. - Princeton NJ

B01L 300

422102, 422 681, 422 99, 422100, 436174, 436179, 436180

Multiple fluid sample processors and systems for high throughput chemical synthesis and biological assays and/or processing. A multi-layered fluidic array having microchannels, reservoirs and reaction wells is subject to robotic and automated handling. A pressure pumping system is utilized for fluid delivery and control through the synthesis process. The sizes of the micro-sized channels, apertures, and valves are adjusted to optimize fluid distribution and channel filling. The fluid sample processors can be grouped together in a microtiter format to increase the speed, quantity and efficiency of the processing.

6720143, Apr 13, 2004

Apr 27, 2001

09/844980

Robert D. Juncosa - Yardley PA
Rene Bongard - Princeton NJ
Johannes Dapprich - Lawrenceville NJ
Richard Scribner - Shingle Springs CA

Orchid BioSciences, Inc. - Princeton NJ

C12Q 168

435 6, 4352831, 4352872, 4352883, 4352885, 422 50, 422 58, 422 681

A genetic analysis device particularly for determining the presence or absence of Single Nucleotide Polymorphisms (SNPs) within specific sequences of DNA. The device includes a housing, at least one glass slide member, and an elastomeric member with channels thereon. Oligo arrays are spotted on the glass slide member(s) and subjected to DNA samples, reagents or the like. A plurality of openings or ports allow entry of samples, reagents or wash materials, while a plurality of exit ports or openings allow removal of such materials. The assay devices can be used for multiple samples or a single sample. A plurality of synthesis devices can be positioned in a support base in order to allow sampling in an automated manner. The synthesis devices can be provided in a 96 well microtiter format.

7618811, Nov 17, 2009

Feb 24, 2005

10/906546

Robert Juncosa - Fairport NY, US
Joel Grover - Pittsford NY, US

Thermal Gradient - Pittsford NY

C12M 1/34

4352872, 4352885, 4352887, 4352931, 4353031

A multi-layer device suitable for thermal cycling is set forth. The device is particularly suitable for performing a polymerase chain reaction (PCR). One embodiment includes a first layer (), a second layer () adjacent to the first layer, and a third layer () adjacent to the second layer opposite the first layer. A continuous channel () is formed within the first layer (), the second layer (), and the third layer (). The continuous channel () has a plurality of cycle segments, each of the cycle segments comprises a first portion () disposed within the first layer (), a second portion () disposed within the third layer (), and a third portion () disposed within the second layer (). An integral or separate temperature source or sources (), () may be provided to maintain the first layer () at a first temperature and the third layer () at a second temperature. Thermal conduction maintains the third layer () at a temperature between the first temperature and the second temperature.

7704726, Apr 27, 2010

Aug 17, 2006

11/505679

Michael James Heller - Encinitas CA, US
James Patrick O'Connell - Del Mar CA, US
Robert David Juncosa - Mission Viejo CA, US
Ronald George Sosnowski - Coronado CA, US
Thomas Ratcliffe Jackson - San Diego CA, US

Gamida For Life B.V. - Rotterdam

C12M 1/36
G01N 15/06

4352831, 4352872, 422 681, 422 8201

A system for performing molecular biological diagnosis, analysis and multistep and multiplex reactions utilizes a selfaddressable, selfassembling microelectronic system for actively carrying out controlled reactions in microscopic formats. The device includes a power supply and waveform generator adapted to supply a DC bias and superimposed AC signal to the system through an interface to the array of microlocations.

20010052976, Dec 20, 2001

Aug 9, 2001

09/927820

Robert Juncosa - Mission Viejo CA, US
William Butler - Carlsbad CA, US
Lei Wu - San Diego CA, US
Robert Cormack - Boulder CO, US

Nanogen, Inc. - San Diego CA

C12Q001/68
G01J003/36

356/307000, 435/006000

An optical detection system is adapted particularly for detection of biological reactions, especially fluorescent or chemilluminescent reactions. An excitation source, preferably a laser, illuminates a portion of an object to be examined, the portion preferably comprising one microlocation out of an array of microlocations. An intervening optical detection platform serves to direct the excitation radiation, preferably through use of a scanning system, most preferably through use of an x-y mirror-based scanning system to the portion of the object to be illuminated. A detector, preferably a photomultiplier tube, receives the emitted radiation from the objects to be examined, the detector being characterized in that the diameter of the region examined by the detector is the same as or smaller than the diameter of the illuminated region, and comprises less than the entire surface of the object to be examined, and most preferably images a whole or a part of a single microlocation. Preferably, the excitation source is coupled to the optical detection platform via an optical fiber. In operation, a confocal microscopy system is formed in which the excitation radiation is substantially in focus at the surface of the object to be examined, the excitation radiation having a lateral extent less than the entire diameter of the object to be examined and the detection system having a lateral field of view of a diameter substantially the same as or less than the diameter of the excitation region. In one aspect of this invention, the optical detection platform includes an excitation detector which measures reflected excitation radiation from the object to be examined. This information is then compared to prestored information regarding the location of the microlocations and interstitial regions on the object to be examined, whereby alignment information is obtained. The excitation radiation may then be precisely directed to a given microlocation or portion thereof so as to perform the examining through the confocal system. Significant increases in signal-to-noise ratio are achieve.

20090197306, Aug 6, 2009

Feb 9, 2009

12/367649

Robert Juncosa - Fairport NY, US
Joel Grover - Pittsford NY, US

C12P 19/34

435 912

A multi-layer device suitable for thermal cycling is set forth. The device is particularly suitable for performing a polymerase chain reaction (PCR). One embodiment includes a first layer (), a second layer () adjacent to the first layer, and a third layer () adjacent to the second layer opposite the first layer. A continuous channel () is formed within the first layer (), the second layer (), and the third layer (). The continuous channel () has a plurality of cycle segments, each of the cycle segments comprises a first portion () disposed within the first layer (), a second portion () disposed within the third layer (), and a third portion () disposed within the second layer (). An integral or separate temperature source or sources (), () may be provided to maintain the first layer () at a first temperature and the third layer () at a second temperature. Thermal conduction maintains the third layer () at a temperature between the first temperature and the second temperature.

20110244466, Oct 6, 2011

Apr 1, 2011

13/078282

Robert Juncosa - Fairport NY, US
Joel Grover - Pittsford NY, US

C12Q 1/68
C12M 1/10
C12M 1/34
C12P 19/34

435 612, 4352891, 4352872, 435 912

Devices and systems for extracting, purifying and amplifying nucleic acids, and methods for use of such devices and systems. The devices have top sections which include a plurality of syringe vessels with applicable reagent materials, as well as a channel for a specimen collection dropper. Plungers force the materials sequentially into reaction chambers in the middle sections. Once the samples are extracted and purified, they are drawn by a vacuum into PCR devices where they are subjected to denaturing, annealing and extension steps and amplified. Interrogation of flow cells provide real-time quantitative detection.

62251090, May 1, 2001

May 27, 1999

9/321170

Robert D. Juncosa - Yardley PA
Rene Bongard - Princeton NJ
Johannes Dapprich - Lawrenceville NJ
Richard Scribner - Shingle Springs CA

Orchid BioSciences, Inc. - Princeton NJ

C12M 136
C12Q 168
G01N 1506
G01N 3122

4352885

A genetic analysis device particularly for determining the presence or absence of Single Nucleotide Polymorphisms (SNPs) within specific sequences of DNA. The device includes a housing, at least one glass slide member, and an elastomeric member with channels thereon. Oligo arrays are spotted on the glass slide member(s) and subjected to DNA samples, reagents or the like. A plurality of openings or ports allow entry of samples, reagents or wash materials, while a plurality of exit ports or openings allow removal of such materials. The assay devices can be used for multiple samples or a single sample. A plurality of synthesis devices can be positioned in a support base in order to allow sampling in an automated manner. The synthesis devices can be provided in a 96 well microtiter format.