Inventors:
Samuel Nochumson - The Woodlands TX, US
Ross Durland - The Woodlands TX, US
Audrey Yu-Speight - Austin TX, US
John Welp - Willis TX, US
Kuowei Wu - Houston TX, US
Rexford Hayes - Milford MA, US
International Classification:
C12Q 1/68
C02F 1/02
B01D 15/00
Abstract:
A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinant cells. Effective, controllable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and neutralization.