Inventors:
Randolph R. Henke - Knoxville TN
Assignee:
Agrigenetics Research Associates Limited - Boulder CO
International Classification:
A01G 100
Abstract:
A method is disclosed which selects for different types of mutants expressed in cereal seed. The method allows rapid qualitative or quantitative screening of the aleurone GA. sub. 3 -induced expression of alpha-amylase activity in half-seeds (without embryo). Half-seeds of the M. sub. 2 population of mutagenized cereal seeds are incubated under selection pressures that normally inhibit the expression of alpha-amylase activity. These selection agents include metabolic inhibitors which may function at the level of transcription, translation, or protein secretion. These selection agents include antimetabolites such as amino acid analogs (e. g. , S-2-aminoethylcysteine, p-flourophenylalaine, canavanine, etc. ), antibiotics (e. g. Actinomycin D, cycloheximide, erythromycin, kanamycin, streptomycin, or tunicamycin), biocides, herbicides, water or salt stress.