Inventors:
Dennis J. Gray - Howey-in-the-Hills FL, US
Jayasankar Subramanian - Tavares FL, US
Richard E. Litz - Homestead FL, US
Assignee:
University of Florida Research Foundation, Inc. - Gainesville FL
International Classification:
A01H 1/00
A01H 5/00
A01H 5/08
C12N 5/04
US Classification:
800279, 800295, 800298, 435430, 4354301, 435418, 435419
Abstract:
The invention features a method of selecting for a grape somatic embryo or grape plant having resistance to a plant pathogen using a liquid culture medium. The method including the steps of (a) culturing a grape embryonic culture in a first liquid culture medium to grow a cellular suspension culture, the first liquid culture medium including a plant growth regulator and a filtrate from a plant pathogen culture; (b) recovering an embryogenic cell or embryogenic cell mass from the cellular suspension culture; and (c) culturing the embryogenic cell or embryogenic cell mass in a second liquid culture and in a third culture media to select a grape somatic embryo or a grape plant having resistance to a plant pathogen.