Inventors:
David Lederman - Morgantown WV, US
Daniel Flynn - Morgantown WV, US
Peter Mico Gannett - Morgantown WV, US
Odille Cue Noriega Myers - Morgantown WV, US
International Classification:
G01N 33/553
B01J 19/00
B05D 1/36
G01N 33/00
B05D 7/24
G03F 7/00
US Classification:
436525, 422 681, 427402, 427414, 4274191, 427404, 430296, 436 94, 436 86
Abstract:
A microcantilever sensor for targeted analyte detection can generally comprise a microcantilever having a base and a beam, a metallic coating disposed substantially only on a first surface of a distal-most end of the beam, and a receptor compound immobilized to the metallic coating wherein the receptor compound can have substantially exclusive binding interaction with the analyte. The receptor compound can be a thiol-terminated bifunctional compound having a receptor site with specific binding affinity for the analyte, for example, an isolated Fab′ fragment. The metallic coating can be a noble metal and/or a semi-noble metal, such as a bilayer of chromium and gold. The metallic coating can be applied to the microcantilever surface by electron-beam lithography.