Paul J Brynes

48974447, Jan 30, 1990

Jun 3, 1985

6/740706

Paul J. Brynes - Libertyville IL
Patricia Andrade-Gordon - Port Jefferson NY

The Research Foundation of the State University of New York - Albany NY

C08L 8900

525 541

An immobilized fluorogenic substrate useful in processes for identifying and quantifying, intra- and extra-cellularly, and mammalian body fluids, as well as animal cell abstracts is disclosed. The immobilized fluorogenic substrate has the structure R. sub. 1 --NH--R. sub. 4 --R. sub. 2 --R. sub. 3 wherein R. sub. 1 represents an enzyme-specific oligopeptide; R. sub. 2 represents a spacer group which is methylenecarbonyloxy, a methylenecarboxamido, or a methylanesulfonamido group attached to a polymethylene chain which itself has a functional group suitable for coupling with a polymer; R. sub. 3 represents a biologically inert polymer; and R. sub. 4 represents a fluorogenic moiety.

52487911, Sep 28, 1993

Jan 14, 1992

7/820729

Paul J. Brynes - Libertyville IL
Donald D. Johnson - Lindenhurst IL
Cynthia M. Molina - Deerfield IL
Charles A. Flentge - Lake Villa IL
Patrick F. Jonas - Waukegan IL

Abbott Laboratories - Abbott Park IL

C07D31182

549223

A fluorescence polarization immunoassay (FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer/antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents.

49523368, Aug 28, 1990

Jul 22, 1989

7/223193

Paul J. Brynes - Libertyville IL
Cynthia M. Molina - Northbrook IL
Janis A. Martinus - Wildwood IL
Kenward S. Vaughan - Carlsbad CA
Catherine M. Smith - Carlsbad CA

Abbott Laboratories - Abbott Park IL

C09K 1106

25230116

This disclosure relates to a method and reagents for determining amphetamine and methamphetamine in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of amphetamine and methamphetamine in a single assay and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described includes pretreatment of the biological sample to eliminate cross-reactants such as. beta. -hydroxyphenethylamine by preincubating the sample solely with an aqueous periodate solution having a pH from about 4. 0 to about 7. 5 without adjustment to an alkaline pH.

53546938, Oct 11, 1994

Jun 29, 1993

8/083928

Paul J. Brynes - Libertyville IL
Donald D. Johnson - Lindenhurst IL
Cynthia M. Molina - Deerfield IL
Charles A. Flentge - Lake Villa IL
Patrick F. Jonas - Waukegan IL

Abbott Laboratories - Abbott Park IL

G01N 33542

436537

A fluorescence polarization immunoassay (FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer-antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents.

51010151, Mar 31, 1992

Apr 10, 1989

7/335627

Paul J. Brynes - Libertyville IL
Donald D. Johnson - Lindenhurst IL
Cynthia M. Molina - Deerfield IL
Charles A. Flentge - Lake Villa IL
Patrick F. Jonas - Waukegan IL

Abbott Laboratories - Abbott Park IL

G01N 33536
C09K 1106

530363

A fluorescence polarization immunoassay (FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer/antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents.