Patricia M Scensny

53976959, Mar 14, 1995

Jun 29, 1989

7/373304

Richard C. Sutton - Irondequoit NY
Susan J. Danielson - Rochester NY
Pranab Bagchi - Webster NY
Patricia M. Scensny - Rochester NY

Eastman Kodak Company - Rochester NY

C12Q 170
G01N 33543
C07K 300
C12N 1108

435 5

Useful materials for diagnostic tests, affinity chromatography, enzymatic reactions and immunoassays are prepared by covalently attaching reactive compounds containing reactive amino or sulfhydryl groups to polymeric particles having pendant carboxyl groups on the outer surfaces. Such reactive compounds include biologically reactive species, such as enzymes, polypeptides and proteins. This attachment is carried out using carbamoylonium compounds which react with the carboxyl groups to form intermediate reactive groups which then react with the amino or sulfhydryl groups to form a covalent linkage between particle and reactive compound. A kit comprises polymeric particles having carboxyl groups on the outer surfaces, and a carbamoylonium compound.

52665003, Nov 30, 1993

Aug 3, 1989

7/389390

Patricia M. Scensny - Rochester NY
Chung-yuan Chen - Rochester NY

Eastman Kodak Company - Rochester NY

G01N 33546

436534

A method for attaching a reactive amine- or sulfhydryl-containing compound to polymeric particles using dication ether salts is disclosed.

49524950, Aug 28, 1990

Jun 8, 1987

7/059667

Robert T. Belly - Ithaca NY
Patricia M. Scensny - Rochester NY
Annie L. Wu - Penfield NY
Chung-yuan Chen - Rochester NY

Eastman Kodak Company - Rochester NY

C12Q 134
C07D24146

435 18

Hydrolyzable substrates comprise blocked moieties which, when cleaved from the substrate during hydrolysis, provide electron transfer agents. The released electron transfer agents can be recycled between a reductant and a reducible compound that upon reduction provides a detectable species. Alternatively, they can be recycled between an oxidant and an oxidizable compound that upon oxidation provides a detectable species. These substrates are useful in analytical compositions, elements and methods for the determination of hydrolytic analytes, such as hydrolytic enzymes or biological cells containing such enzymes.

48124095, Mar 14, 1989

Jan 31, 1986

6/824756

Bruce E. Babb - Rochester NY
Robert T. Belly - Webster NY
Patricia M. Scensny - Rochester NY

Eastman Kodak Company - Rochester NY

G01N 3353

435 7

Hydrolyzable substrates comprise blocked dye moieties which, when cleaved from the substrate during hydrolysis, provide fluorescent dyes having maximum absorptions above about 530 nm and maximum emissions at least about 580 nm at physiological pH. These substrates can be used in analytical determinations of hydrolytic substances including hydrolytic enzymes or biological cells containing such enzymes.

47466073, May 24, 1988

Feb 7, 1985

6/699374

Albert J. Mura - Rochester NY
Patricia M. Scensny - Rochester NY
Vanessa R. Lum - Rochester NY
Robert T. Belly - Webster NY

Eastman Kodak Company - Rochester NY

C12Q 102
C12Q 126
G01N 3300
G01N 3350

435 25

Certain substituted benzo- and naphthoquinone electron transfer agents are useful in analytical compositions, elements and methods, e. g. for determinations of living cells. These electron transfer agents are capable of being reduced by an analyte, and the reduced electron transfer agent, in turn, reduces another compound providing a detectable species (e. g. a dye). The reduction potential (E. sub. 1/2) of the electron transfer agents useful inthis invention is in the range of from about -320 to about +400 mV as measured in an aqueous buffer solution at pH 7.