Inventors:
E. Premkumar Reddy - Villanova PA, US
M. V. Ramana Reddy - Upper Darby PA, US
Natarajan Dhanasekaran - Bryn Mawr PA, US
Assignee:
Temple University-of the Commonwealth System of Higher Education - Philadelphia PA
International Classification:
C12N009/00
C07H021/04
US Classification:
435 25, 435 4, 435 6, 435183, 435189, 4352523, 4353201, 435325, 536 232, 536 237, 514789
Abstract:
A cell-based assay is provided for screening substances for the capacity to inhibit COX-2 activity. The assay utilizes as a screening reagent a cell line which has been engineered to achieve constitutive or inducible expression of COX-2. In one embodiment, the cells express a GTPase-deficient, constitutively-activated mutant form of the α-subunit of protein G12 which induces the production of arachidonic acid and COX-2. Decreased cell proliferation in the presence of test substance indicates that the substance has COX-2 inhibitory activity.