Michelle T Lane

6762053, Jul 13, 2004

Jun 8, 2001

09/877395

David K. Gardner - Highlands Ranch CO
Michelle T. Lane - Highlands Ranch CO

Vitrolife, Inc. - Englewood CO

C12N 500

435407, 435325, 435404

The present invention provides a supplement and a culture media useful for culturing mammalian gametes and embryonic tissue. The culture media comprises at least one of recombinant human albumin, fermented hyaluronan, and citrate. Because the constituents are produced from non-conventional sources, the culture medium is free from contaminants such as viruses, prions and endotoxins. Additionally, because the medium is completely defined, the medium is not subject to variations which can impair the development of mammalian cells and prevent meaningful comparisons of empirical studies.

6838235, Jan 4, 2005

Dec 18, 2002

10/322914

David K. Gardner - Highlands Ranch CO, US
Michelle Lane - Highlands Ranch CO, US

Vitrolife Group - Gothenburg

A01N 102
C12N 508

435 2, 435366, 435374, 435404

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer; and cryopreservation.

7087370, Aug 8, 2006

Sep 10, 2002

10/241061

Katrina T. Forest - Madison WI, US
Michelle T. Lane - Littleton CO, US

A01N 1/00

435 13, 435260, 4353071, 435975

The present invention relates to systems for the vitrification of a biological specimen. The systems employ a transfer instrument such as a loop, containing a base medium that includes a cryoprotectant. The biological specimen which has undergone vitrification may be stored for a period of time, and then thawed at a later date. The thawed biological specimen remains viable. Preferred biological specimens according to the present invention are developmental cells.

20020115054, Aug 22, 2002

Oct 13, 1999

09/416992

KATRINA FOREST - MADISON WI, US
MICHELLE LANE - LITTLETON CO, US

A01N001/00
A01N001/02

435/001300

The present invention relates to a method of vitrification of a biological specimen. According to the method of the present invention, a biological specimen is directly exposed to a freezing material. Upon exposure to the freezing material, the biological specimen undergoes vitrification. The biological specimen which has undergone vitrification may be stored for a period of time, and then thawed at a later date. The thawed biological specimen remains viable. Preferred biological specimens according to the present invention are developmental cells.

20060234204, Oct 19, 2006

Mar 24, 2006

11/389552

Katrina Forest - Madison WI, US
Michelle Lane - Littleton CO, US

A01N 1/02

435001300, 435002000

The present invention relates to a method of vitrification of a biological specimen. According to the method of the present invention, a biological specimen is directly exposed to a freezing material. Upon exposure to the freezing material, the biological specimen undergoes vitrification. The biological specimen which has undergone vitrification may be stored for a period of time, and then thawed at a later date. The thawed biological specimen remains viable. Preferred biological specimens according to the present invention are developmental cells.

20060292688, Dec 28, 2006

Apr 7, 2006

11/400746

David Gardner - Highlands Ranch CO, US
Michelle Lane - Littleton CO, US
Peter Svalander - Hilnlycke, SE

C12N 5/06

435325000, 435404000

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer, and cryopreservation.