Martin B Bigos

6673556, Jan 6, 2004

Sep 12, 2001

09/954392

Douglas Nixon - San Francisco CA
Adrian B. McDermott - Leyburn, GB
Scott Furlan - San Francisco CA
Martin Bigos - San Francisco CA
Megan Sheehy - Syracuse NY
Paul Klenerman - Oxford, GB

The J. David Gladstone Institutes - San Francisco CA

G01N 3353

435 72, 435 405, 435 4, 435 721

The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.

7186516, Mar 6, 2007

Oct 29, 2003

10/697737

Douglas Nixon - San Francisco CA, US
Adrian B. McDermott - Leyburn, GB
Scott Furlan - San Francisco CA, US
Martin Bigos - San Francisco CA, US
Megan Sheehy - Syracuse NY, US
Paul Klenerman - Oxford, GB

The J. David Gladstone Institutes - San Francisco CA

G01N 33/567
C12Q 1/00

435 721, 435 4

The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.

20050203976, Sep 15, 2005

Feb 9, 2005

11/055424

William Hyun - Tiburon CA, US
Martin Bigos - San Francisco CA, US
Antony Chiang - San Francisco CA, US
Lawrence Roberts - San Francisco CA, US

G06F017/30

707204000

Methods, tools and systems for efficiently and intuitively transferring files from client computers, via a network, to a centralized location for storage of the files as well as storage of metadata associated with the files in a manner such that the metadata as well as the files may be searched in the database by users to identify files of interest.

20050223024, Oct 6, 2005

Mar 30, 2005

11/096011

William Hyun - Tiburon CA, US
Martin Bigos - San Francisco CA, US
Antony Chiang - San Francisco CA, US
Lawrence Roberts - San Francisco CA, US

G06F017/00

707102000

Methods, systems and computer readable media for users of a shared database, file system, or other similar software system to browse files or records in the database according to any of the files' attributes in a standard hierarchical tree structure. Methods, systems and computer readable media that enable users to dynamically define and customize a hierarchy of files according to the quantity and priority of attributes that each user individually desires, such that the records or files are automatically rearranged and reorganized into the user-created hierarchy.

6178382, Jan 23, 2001

Jun 23, 1998

9/103284

Mario Roederer - Redwood City CA
Adam S. Treister - San Carlos CA
Martin Bigos - San Francisco CA
David R. Parks - San Francisco CA
Wayne A. Moore - San Francisco CA
Leonore A. Herzenberg - Stanford CA

The Board of Trustees of the Leland Stanford Junior University - Stanford CA

G01N 3350
G06F 1900

702 21

This invention relates a series of methods for the visual representation and subsequent application of analyses on complex data sets. In particular, this invention is useful for the analysis of multiple sample sets that share common features, for which similar types of analyses are desired. Three concepts are embodied to aid in this analysis: "Functional equivalence by Algorithmic Polymorphism" (FEAP), in which an analysis algorithm is abstracted through the use of an associated name; a genealogical metaphor for the representation of successive or parallel analysis steps, in which "families" of analyses can be easily copied between different sample data sets; and batch analysis through the creation of multi-sample analysis surrogates (MSAS), which are groups of samples wherein analyses applied to an MSAS is then applied to every sample within the MSAS group. This invention has particular utility in the analysis of data derived from flow cytometers, the analysis of complex demographic data, and other similar data sets.