Mark A Villamil

University of Delaware

Sep 2008 to 2000
Research Assistant
The Richard Stockton College of New Jersey
Pomona, NJ
Sep 2009 to May 2010
Teaching Assistant
James Wingrave, Department of Chemistry and Biochemistry

Feb 2009 to May 2009
Graduate TA Mentor
Mary Beth Kramer, Department of Chemistry and Biochemistry

Sep 2008 to Dec 2008
Instructor
The Richard Stockton College of New Jersey

May 2006 to May 2007
Researcher

University of Delaware
Newark, DE
Sep 2008 to 2000
Ph. D. in Biochemistry
The Richard Stockton College of New Jersey
May 2007
School of Natural Sciences and Mathematics
Bachelor of Science in Biochemistry and Molecular Biology

Research Skills Extensive experience in biochemistry and molecular biology An in-depth understanding of mechanistic enzymology, steady-state kinetics and enzyme inhibition analysis Assay development using fluorescence and gel based techniques Molecular biology techniques for sub-cloning, mutagenesis, deletion and insertion of specific protein sequences and tags, unnatural amino acid incorporation Recombinant protein expression in insect cells (Sf9) and bacterial cells Culturing of HEK293T cells Biochemistry techniques including SDS-PAGE, native gel electrophoresis, pull- down assays, Western blotting, chemical cross-linking, and in-gel digestion Protein purification using affinity tags (GST and 6xHis), conventional columns, ion exchange, and size exclusion Extensive knowledge in the usage of Fluoromax-4 fluorescence spectrophotometer, thermal cycler, AKTAFPLC and DuoFlow Mass spectrometry of protein and peptides. In-gel and in-solution protein digestion. Peptide and protein identification by Mascot data base search. Hydrogen-deuterium exchange (HDX) analysis Computer software: Origin, GraphPad Prism, PyMOL, Swiss-Model, and Illustrator