Inventors:
Shuliang Zhang - Miami FL, US
Jiuliu Lu - Homestead FL, US
Min Zheng - Pembroke Pines FL, US
Eric M. Grace - Cooper City FL, US
Jing Li - Miami FL, US
Maritza Lavernia - Miami FL, US
Ted W. Britton - Sunrise FL, US
Assignee:
Beckman Coulter, Inc. - Brea CA
International Classification:
G01N 33/72
G01N 33/48
US Classification:
436 66, 436 8, 436 10, 436 63, 436164, 436174, 436175, 436149, 436150, 422 8202, 422 8205, 422 8209, 422 73, 435 2, 435 29
Abstract:
A method of correction of particle interference to hemoglobin measurement of a blood sample on a hematology analyzer is provided. The method includes mixing an aliquot of a blood sample with a lytic reagent to lyse red blood cells and forming a sample mixture; measuring absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed in the sample mixture, and obtaining an apparent hemoglobin concentration of the blood sample using obtained absorbance; measuring concentration and size of cellular particles remaining in the sample mixture; removing contribution of the cellular particles to the apparent hemoglobin concentration using the concentration and the size of the cellular particles to obtain a corrected hemoglobin concentration of the blood sample; and reporting the corrected hemoglobin concentration of the blood sample.