Inventors:
Maris V. Fonseca - Madison WI
Assignee:
Wisconsin Alumni Research Foundation - Madison WI
International Classification:
C12P 1942
Abstract:
It is disclosed that the enzymes ATP:corrinoid adenosyltransferase (CobA) from and reduced flavodoxin (FldA) from are both necessary and sufficient, in the presence of ATP, to reduce the oxidation state of the cobalt atom in cobalamin and produce adenosylcobalamin. This makes possible the construction of plasmids to confer upon microbial fermentation hosts the ability to synthesize adenosylcobalamin, the supply of which can sometimes be a limitation on microbial growth in fermentation. Since the enzyme flavodoxin reductase (Fpr), also from , can act to reduce FldA for biological activity, that enzyme should preferably be expressed in a fermentation host as well.