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Marina Y Komarovskaya

from Milford, CT
Age ~62
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Also known as:
Marina E Komarovskaya, Marina L Karkanitsa, Marina A
Phone and address:
78 Stagecoach Cir, Milford, CT 06460
(203) 809-3797
Connected to:
Maksim S Komarov, 40Vera Y Komarova, 50Jennifer L Komas, 54Pamela Komas, 59Amanda E Komaschka, 45Aleksey M Komashko, 50Rahul Komati, 35Prasanna Komatireddy, 44Christine L Komatsu, 40Kristin M Komatsubara, 42

Marina Komarovskaya Phones & Addresses

  • 78 Stagecoach Cir, Milford, CT 06460 (203) 809-3797
  • Philadelphia, PA
  • Wynnewood, PA
  • Shelton, CT
  • 27 Earle St, Milford, CT 06460

Professional Records

License Records

Marina Komarovskaya

Address:
27 Earle St, Milford, CT 06460
License #:
107536 - Active
Category:
Registered Nurse
Issued Date:
Aug 13, 2012
Expiration Date:
Sep 30, 2017

Publications

Us Patents

Transient Transfection With Rna

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US Patent:
20080260706, Oct 23, 2008
Filed:
Feb 4, 2008
Appl. No.:
12/025700
Inventors:
Peter M. Rabinovich - Madison CT, US
Sherman M. Weissman - Milford CT, US
Marina E. Komarovskaya - Milford CT, US
Erkut Bahceci - Hamden CT, US
International Classification:
A61K 35/12
C12N 15/11
C12P 19/34
C12N 5/06
A61P 43/00
C12N 15/87
A61K 31/70
US Classification:
424 9321, 536 231, 435 912, 435440, 514 44, 424 932, 435377, 435455
Abstract:
A method of mRNA production for use in transfection is provided, that involves in vitro transcription of PCR generated templates with specially designed primers, followed by polyA addition, to produce a construct containing 3′ and 5′ untranslated sequence (“UTR”), a 5′ cap and/or Internal Ribosome Entry Site (IRES), the gene to be expressed, and a polyA tail, typically - bases in length. This RNA can efficiently transfect different kinds of cells. This approach results in increased efficiency (fidelity and productivity) of mRNA synthesis and is less time consuming because it does not require cloning, and also consequently eliminates the unwanted errors and effects related to RNA made on DNA templates obtained with cloning techniques. The results of transfection of RNAs demonstrate that RNA transfection can be very effective in cells that are exceedingly difficult to transfect efficiently with DNA constructs. Further, the levels of gene expression following mRNA transfection are consistent from cell to cell in an experiment and these levels can be controlled over a wide range simply by changing the amount of mRNA that is transfected, and without obvious cytotoxic effects due to the levels of RNA per se. Due to high efficiency the cells can be simultaneously transfected with multiple genetic constructs. The method can be used to deliver genes into cells not- or only poorly transfectable for DNA, in vitro and in vivo.

Cells Prepared By Transient Transfection And Methods Of Use Thereof

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US Patent:
20160151491, Jun 2, 2016
Filed:
Jan 27, 2016
Appl. No.:
15/008376
Inventors:
- New Haven CT, US
Sherman M. Weissman - New Haven CT, US
Marina E. Komarovskaya - Milford CT, US
Erkut Bahceci - Hamden CT, US
Samuel Katz - Woodbridge CT, US
Efim Golub - East Haven CT, US
International Classification:
A61K 39/395
A61K 35/12
A61K 45/06
C12N 15/85
Abstract:
Compositions and methods of making cells using RNA, and cells made using the disclosed compositions and methods are also provided. In exemplary embodiments, RNA is transfected into cells to effect a molecular, biological, physiological, or histological change in the cells. In preferred embodiments, the RNA is prepared in vitro, more preferably using a DNA template according to the provided compositions and methods. Methods for treating or inhibiting a disorder or disease such cancer are also provided. The methods can include, for example, locally or systemically administering to the host an effective amount of one or more RNAs; or an effective amount of population of cells isolated from the subject or a syngeneic or histocompatible subject, contacted ex vivo with one or RNAs, and optionally expanded. The cells can be, for example, immune cells or stem cells.
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Marina Y Komarovskaya from Milford, CT, age ~62 Get Report