Joseph A Yourno

55567738, Sep 17, 1996

Aug 18, 1994

8/292524

Joseph Yourno - Slingerlands NY

C12P 1934
C12Q 168
C12Q 170
C12N 1102

435 912

A nested polymerase chain reaction (PCR) performed in a single reaction tube that remains closed after the reaction mixtures for each amplification have been introduced therein. The reaction mixture for the second PCR amplification is sequestered and preserved in an upper portion of the single, closed reaction tube during the first amplification, and subsequently introduced into the reaction space containing the end product of the first PCR amplification, without opening the reaction tube.

54320976, Jul 11, 1995

Nov 9, 1993

8/149543

Joseph Yourno - Slingerlands NY

G01N 100

436175

White blood cells (WBC) were recovered from dried blood spots on Guthrie cards by a simple and efficient prototypic method. The mummified formed elements of dried blood on filter paper are first fixed, then the red blood cells laked in-situ. Last, the paper matrix is itself digested away with cellulase in acid buffer, releasing the partially rehydrated, mummified WBC and other cellular materials into suspension for collection and examination.