Joseph Anthony Mamone

8470153, Jun 25, 2013

Jul 22, 2011

13/188584

Marc N. Feiglin - East Brunswick NJ, US
Joseph Mamone - Hillsborough NJ, US
Anne R. Kopf-Sill - Portola Valley CA, US
Ian Fitzpatrick - Elwood Victoria, AU
Mark Rob - East Bentleigh Victoria, AU
Phillip Duncan - Mount Waverley Victoria, AU

Tecan Trading AG - Mannedorf

C02F 1/04
C02F 11/00
C25B 9/00
C25B 11/00
C25B 13/00
G01N 27/00

204600, 204450, 422512, 422547, 422551, 422552

A cartridge () comprises a working film () for manipulating samples in liquid droplets with an electrode array () when the working film () of the cartridge () is placed on said electrode array (). The cartridge () comprises a body (″) with a number of wells () configured to hold therein reagents () or samples (′); a flexibly deformable top structure () impermeable to liquids and configured to seal a top side of the wells (); a piercable bottom structure () impermeable to liquids and configured to seal a bottom side of the wells (); a working film () located below a lower surface () of the body (″), the working film () being impermeable to liquids and comprising a hydrophobic upper surface (); a peripheral spacer (″) located below the lower surface () of the body (″) and connecting the working film () to the body (″); a gap () between the lower surface () of the body (″) and the hydrophobic upper surface () of the working film (), the gap () being defined by the peripheral spacer (″); and a number of piercing elements () located below piercable bottom structures () and configured to pierce the piercable bottom structures () for releasing reagents or samples (′) from the wells () into the gap (). Also disclosed is a system () with an electrode array () onto which the cartridge () can be placed.

20130095474, Apr 18, 2013

Oct 13, 2011

13/272961

JOSEPH MAMONE - Hillsborough NJ, US
Howard Gamper - Philadelphia PA, US

C12Q 1/68
G01N 21/64
C07H 21/04

435 611, 536 243

Stem-loop probe for single nucleotide polymorphism (SNP) genotyping of individual SNP nucleic acid target sequences has first, second, and third single stranded nucleic acid portions. Second portion is between the first and third portions. First and third portions build a double stranded, intramolecular stem. Second portion forms a single stranded oligonucleotide loop with a nucleotide sequence, complementary to individual SNP nucleic acid target sequences. The nucleotide sequence of probe is matches probe/target hybrids have a melting point Tthat is at least 5 C. higher than Tof mismatched probe/target hybrids. A method of detecting SNP in nucleic acid containing samples uses a pair of stem-loop probes for SNP genotyping of two individual SNP nucleic acid target sequences. The probes comprise the same first, second, and third portions and a ratio of perfect match probe/target hybrids to mismatched probe/target hybrids is detected at a certain temperature.

20130175169, Jul 11, 2013

Mar 4, 2013

13/784168

TECAN TRADING AG - Mannedorf, CH
Michael Benjamin Franklin - Claremont CA, US
Timothy Francis Lee - Forest Hill, AU
Joseph Mamone - Hillsborough NJ, US

TECAN TRADING AG - Mannedorf

G01N 27/447

204450, 204600

Liquid droplet manipulation instrument has electrode array for inducing movement of a droplet by electrowetting, substrate supporting the array and control with electrode selector connected to a voltage control. The selector selects each electrode and provides each with a controlled voltage. The control includes central processing unit for providing the selected electrode with an individual voltage pulse which is a drive voltage or a ground voltage or a stop voltage. The control defines a path for movement of a liquid portion of a larger volume that covers more than one electrode by the simultaneous selection of a group of two or more subsequent drive electrodes and to provide each selected drive electrode with a drive voltage pulse along the path. The control simultaneously provides a group of two or more electrodes adjacent to or identical with the pulsed drive electrodes, with a ground or stop voltage pulse.

20130183666, Jul 18, 2013

Jan 18, 2012

13/352742

Marc N. Feiglin - East Brunswick NJ, US
Joseph Mamone - Hillsborough NJ, US
Howard Gamper - Philadelphia PA, US

C12Q 1/68

435 611

In a method of detecting the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, the genomic sample is amplified by PCR and a single stranded target DNA is selected and separated for use in differential hybridization experiments. Subsequent partial genotyping comprises the steps of admixing to the target DNA at least one fluorescent labeled STR probe oligonucleotide and one different fluorescent labeled reference probe oligonucleotide allowing hybridization to the single stranded target DNA in a hybridization experiment. Measurement of the fluorescence intensities of the probes that are bound to the repeat units of the selected STR and normalizing the fluorescence intensity of the STR probes on the base of the reference probe intensity reveals a relative fluorescence signal representing the result of the differential hybridization experiment. Also disclosed are kits for carrying out partial genotyping by differential hybridization.

57443122, Apr 28, 1998

Dec 13, 1996

8/766014

Joseph A. Mamone - Parma OH
Maria Davis - Twinsburg OH
Dan Sha - Euclid OH

Amersham Life Science, Inc. - Cleveland OH

C12N 912
C12N 1554
C12P 1934
C12Q 168

435 6

An enzymatically active DNA polymerase or fragment thereof having at least 80% homology in its amino acid sequence to at least a contiguous 40 amino acid sequence of the DNA polymerase of Thermoanaerobacter thermohydrosulfuricus.

58277161, Oct 27, 1998

Jul 30, 1996

8/688649

Joseph A. Mamone - Parma OH

Amersham Life Science, Inc. - Cleveland OH

C12N 912
C12N 922

435194

A Pol-II type DNA polymerase wherein an alanine located at the nucleotide binding site is replaced with a hydroxy containing amino acid.

63331837, Dec 25, 2001

Nov 17, 2000

9/715524

Steven Evans - Gosforth, GB
Joseph Anthony Mamone - Somerset NJ
Maria Davis - Princeton NJ
Bernard A. Connolly - Kingston Park, GB

Amersham Pharmacia Biotech, Inc. - Piscataway NJ

C12N 912
C12N 900
C12P 1934
C07K 100
C07H 2104

435194

Polymerases from the Pol I family which are able to efficiently use ddNTPs have demonstrated a much improved performance when used to sequence DNA. A number of mutations have been made to the gene coding for the Pol II family DNA polymerase from the archaeon Pyrococcus furiosus with the aim of improving ddNTP utilisation. "Rational" alterations to amino acids likely to be near the dNTP binding site (based on sequence homologies and structural information) did not yield the desired level of selectivity for ddNTPs. However, alteration at four positions (Q472, A486, L490 and Y497) gave rise to variants which incorporated ddNTPs better than the wild type, allowing sequencing reactions to be carried out at lowered ddNTP:dNTP ratios. Wild type Pfu-Pol required a ddNTP:dNTP ratio of 30:1; values of 5:1 (Q472H), 1:3 (L490Y), 1:5 (A486Y) and 5:1 (Y497A) were found with the four mutants; A486Y representing a 150-fold improvement over the wild type. A486, L490 and Y407 are on an. alpha.

20150096890, Apr 9, 2015

Dec 16, 2014

14/571914

- Mannedorf, CH
Joseph A. Mamone - Hillsborough NJ, US
Michael Franklin - Claremont CA, US
Timothy Francis Lee - Forest Hill, AU

TECAN TRADING AG - Mannedorf

B01L 3/00
G01N 27/453
B01L 3/02

204450, 204600

A biological sample processing system () includes a liquid droplet manipulation instrument () with an electrode array () for inducing a movement of a liquid droplet () by electrowetting; a substrate (); and a control unit (). An electrode selector () of the control unit () is configured to individually select and provide each electrode () of the electrode array () with a voltage. The control unit () includes a central processing unit () for individually selecting at least one electrode () and for providing the selected electrode(s) () with an individual voltage pulse. The biological sample processing system () also includes a cartridge () with a container ().