John T Kiyasu

43383963, Jul 6, 1982

Aug 13, 1980

6/177662

John Y. Kiyasu - Garden City NY

C12Q 150
B01D 1508

435 17

Improved steps and methods for rapid, efficient and accurate assay of the CPK isozyme MB (cardiac fraction) and other determinations using chromatographic column technique. The improvements are especially adapted for carrying out rapid diagnostic tests in clinics and hospitals. Also contemplated improved apparatus and methods and uses of same adapted for the test method, such apparatus having novel features which can be packaged and merchandised in kit form. Other special features include use of electrophoresis either with or without the conventional chromatographic column analyses and using a selected colored (preferred-green) marker dye. Another special feature is that the used columns of the improved design can be conveniently recycled using the same solutions.

44760167, Oct 9, 1984

Mar 23, 1982

6/360951

John Y. Kiyasu - Garden City NY

B01N 1508

2101982

The invention consists of improved apparatus and a kit packaged improved apparatus and methods for use of such apparatus such kits especially adapted for isolating and identifying and quantitatively measuring the MB iso-enzyme of creatine phosphokinase in fresh human blood serum and apparatus including combinations of racks, glass receptacles and other equipment for making various quantitative and qualitative blood and blood serum tests.

42486424, Feb 3, 1981

Mar 8, 1979

6/018817

John Y. Kiyasu - Garden City NY

B08B 308

134 2

A systematic and programmed wash cycle for cleaning automatic and semi-automatic biochemical analyzers and separators and other equipment. The wash cycle includes a series or sequence of specific and ordered washing steps using a fixed specific sequence of reagents in critical concentrations including use of a hypochlorite reducing agent, a peroxide oxidising agent, an alkaline hydroxide solution, a solution of cetyl pyridinium bromide and cinnamic aldehyde, a solution of a polysulfonated alkyl detergent and a hydrochloric acid solution. It is also contemplated as a special feature, to use a so-called "polishing" system to eliminate from analyzers the micro-organisms and enzymes which cause interference with UV analyses. It is also a particular feature that the ordered wash cycle can be used to clean tubular flow systems, including piping and plumbing, such as may occur in centralized air conditioning units and miscellaneous washing machinery.

41054997, Aug 8, 1978

Oct 6, 1976

5/730102

John Y. Kiyasu - Garden City NY

G01N 3108

1951035R

A diagnostic method for the rapid detection of heart attacks in human patients which comprises passing patient blood serum through a chromatographic column, isolating and identifying the major portion of creatine phosphokinase-MB isoenzyme from the eluate by the inclusion of a green food color. The column may be recycled and reused.