US Patent:
20100160622, Jun 24, 2010
Inventors:
Jean Heather Crinean - Sanford NC, US
International Classification:
C12P 19/04
C07H 11/04
Abstract:
The present invention provides improved methods for producing a solution containing high molecular weight isolated capsular polysaccharides having phosphodiester linkages between saccharide repeat units. In certain methods, COis supplied to a fermentation culture of bacterial cells that produce capsular polysaccharide serotypes containing phosphodiester linkages between saccharide repeat units. Exemplary serotypes containing a phosphodiester linkage between saccharide repeat units include serotypes 6A, 6B, 19A, and 19F. Supplying COto the fermentation culture includes adding bicarbonate ions to the fermentation culture, adding carbonate ions to the fermentation culture, adding mixtures of bicarbonate and carbonate ions to the fermentation culture, and overlaying the fermentation culture with CO.