US Patent:
20040265850, Dec 30, 2004
Inventors:
Hua Wang - Columbus OH, US
Hongliang Luo - Columbus OH, US
Chris Connor - Columbus OH, US
Steven Schwartz - Powell OH, US
Ahmed Yousef - Columbus OH, US
Kai Wan - Columbus OH, US
International Classification:
C12Q001/68
C12P019/34
Abstract:
Tools and methods for detecting the presence bacteria, yeast and mold in a sample obtained from a food sample are provided. The methods employ a polymerase chain reaction and primer and probe sets that are based on the 16S rRNA and squalene-hopene cyclase genes of and and the 18S rDNA gene of mold and yeast. The present invention also relates to primer and probe sets. Each primer and probe set comprises a forward primer and a reverse primer, both of which are from 15 to 35 nucleotides in length and a probe.