Hiroyuki H Yonekawa

20060286623, Dec 21, 2006

Aug 21, 2006

11/438028

Hiroyuki Yonekawa - Dix Hills NY, US

C12M 1/34
C12Q 1/24

435030000, 435287300, 435285100

This invention is used for the three dimensional microanalysis and observation of small object especially under microscope. This invention is applied on to the biomedical field as cell or tissue observation and manipulation. Three dimensional observation and manipulation method and device are cited in claim and . And further cell or tissue manipulating technique as microinjecting, patch clumping and suctioning of living material is cited in claim

20070191715, Aug 16, 2007

Jan 29, 2007

11/698966

Hiroyuki Yonekawa - Bethlehem PA, US

A61B 6/00

600476000

This invention is cited to the method and device for the In Vivo observation of embedded cells or tissues. Using this device, we can observe cells or tissues in inside of living animal for long period with high quality of images. We can couple this device with electro-physiological devices or drug administration devices too. According to this invention, biological reactions of target cells or tissues in inside of animal are observed in real time. Then, arrayed multiplex form of device is also claimed. Such arrayed multiplex device works as high throughput analyzing device In Vitro as well as In Vivo. This device can be used for the drug screening and drug susceptibility test by adding second layer. According to this invention, multiple biological reactions as metastasis of cancer, immunological reaction, drug resistance or host-parasite interaction of target cells can be analyzed at once.

20080171381, Jul 17, 2008

May 2, 2007

11/799487

Hiroyuki Yonekawa - Center Valley PA, US
Osamu Joji - Center Valley PA, US

C12M 3/00

4352872

This invention is used for the multiplex cells/tissues analysis using 3D cells/tissue culturing functional particles. Functional particle has been used for the variety of biological assays including immunology, biochemistry and molecular biology as well as cell biology.We combined different particles in same reaction vessel for realizing multiple assays for cells or tissues analysis. These particles are identified by their mark as different color or fluorescence. Then differently identified cells/tissues are analyzed according to their response to the outside signals evenly. We can apply any signals as chemical trigger, light activation, electric triggers, mechanical trigger or temperature shift. In this patent application, we extend to use these functional particles with permeable parts or permeable reaction vessel for the high trough put cells and tissues analysis. If the diffusion speed of these are calibrated correctly, we can measure complex kinetic of cells or tissues response. Also by the nature of 3D matrix assisted cells/tissues culture technique, we can isolate target cells or tissues mechanically by simple manner after culture or stimuli.

20080261298, Oct 23, 2008

Apr 23, 2007

11/788829

Hiroyuki Yonekawa - Center Valley PA, US

Olympus American Inc. - Center Valley PA

C12M 3/00

4352884

This invention is used for the multiplex analysis method of cells or tissues by combination of multiple cubicles and permeable membrane. Utilizing the diffusion based reagents administration; cells and tissues in multiple cubicles receive signals evenly in their cubicles as parallel manner. This device makes efficient multiplex cells or tissues assays with simple plate layout. According to the molecular permeable mechanism, single spike of stimuli as chemical trigger, light activation, electric triggers or temperature shift can induce multiple cells or tissues response at once. Then accompanying with calibrator molecules as for the navigation of stimuli and assisted by suitable computer simulation, accurate kinetic cells or tissues response analysis is achieved.

20090004110, Jan 1, 2009

Jun 29, 2007

11/823500

Hiroyuki Yonekawa - Bethlehem PA, US

Olympus America Inc. Scientific Equipment Group - Center Valley PA
Olympus Corporation Diagnostic systems group. - Tokyo

C12Q 1/00
A61K 49/00
A61P 43/00

424 91, 435 4

This invention is a continuing submission of multi-functional gelatin particle and its use, provisional patent application 60/880,734 filed at 01/17/2007. And this invention is related to former patent applications of 11/698,966, 11/788,829, 11/799,487. Multi-functional gelatin particle has been used for the variety of biological assays and applications. In this patent application, we extend to use multi-functional gelatin particles as for the in vivo & in vitro shuttle assay.We modified particle preparation steps and then, we can add extra functions in inside and outside of gelatin particles. Such multi-modified and functioned gelatin particles worked for the encapsulation of cells and serve subcutaneous injection too. Such Multi-functional gelatin particles can work as molecular indicator, target molecule isolator, and local area stimulator for the living cells also. Coming from such multi-functionalities, multi-functional gelatin particles will play a carrier of drug delivery into the cells as well as recipient animals. We can utilize every function as molecular attachment, magnetic attraction, fluorescence activation, chemicals release and transformation of its microenvironment altogether. Further we applied simple optical layout, so called tilt angled illumination for the optimized observation tool of gelatin particles when such multi-functional gelatin particle are embedded in the thick medium. Evoked two focal planes by tilt angled illumination, we can observe both side of multi-functional gelatin particle in deeper location of matrix.