Gordon Bentley - Alameda CA, US Henry A. Erlich - Oakland CA, US Russell Gene Higuchi - Alameda CA, US Cherie Holcomb - Oakland CA, US
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
A method for detecting one or more HLA sequence types is described that comprises the steps of: amplifying a plurality of first amplicons from a double stranded nucleic acid sample, wherein the first amplicons are amplified with a plurality of pairs of nucleic acid primers that define exons 2 and 3 of both strands of HLA loci from the group consisting of HLA-A, HLA-B, and HLA-C; amplifying the first amplicons to produce a plurality of populations of second amplicons, wherein each population of second amplicons is clonally amplified from one of the first amplicons; sequencing the plurality of populations of second amplicons to generate a nucleic acid sequence composition for each of the plurality of second amplicons; and detecting variation in the sequence composition from one or more of the second amplicons for one or more of the HLA loci.
High Resolution, High Throughput Hla Genotyping By Clonal Sequencing
Gordon BENTLEY - Alameda CA, US Henry Erlich - Oakland CA, US Russell Higuchi - Alameda CA, US
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
The invention provides methods and reagent for performing full, multi-locus HLA genotyping for multiple individuals in a single sequencing run using clonal sequencing.
Automatic Updating Of Data In Application Programs
- San Francisco CA, US Gordon Bentley - San Jose CA, US
Assignee:
7Layer Technologies - San Francisco CA
International Classification:
G06F 9/445
US Classification:
717171
Abstract:
The present technology includes techniques to generate an application and to regenerate the application when updated content becomes available. While generating the application, a structured dataset can be created to store content for the application. The structured dataset can be manually or automatically edited to update the content. When the software provider is ready to update the application, the application can be regenerated in a single step of receiving the updated version of the structured dataset.
