Frances R Lawyer

57892245, Aug 4, 1998

Jun 2, 1995

8/459383

David H. Gelfand - Oakland CA
Frances C. Lawyer - Davis CA
Susanne Stoffel - El Cerrito CA

Roche Molecular Systems, Inc. - Branchburg NJ

C12N 912

435194

Recombinant DNA sequences encoding the DNA polymerase activity of Thermus thermophilus can be used to construct recombinant vectors and transformed host cells for production of the activity. T. thermophilus DNA polymerase is an. about. 94 kDa protein especially useful in the DNA amplification procedure known as the polymerase chain reaction.

56248334, Apr 29, 1997

Jun 7, 1995

8/475231

David H. Gelfand - Oakland CA
Frances C. Lawyer - Oakland CA
Susanne Stoffel - El Cerrito CA

Hoffmann-La Roche Inc. - Nutley NJ

C12N 912

435194

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

48898180, Dec 26, 1989

Jun 17, 1987

7/063509

David H. Gelfand - Oakland CA
Susanne Stoffel - El Cerrito CA
Frances C. Lawyer - Oakland CA
Randall K. Saiki - Richmond CA

Cetus Corporation - Emeryville CA

C12N 900

435194

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer of non-ionic detergents that lends stability to the enzyme.

48700134, Sep 26, 1989

May 18, 1988

7/196789

David H. Gelfand - Oakland CA
Frances C. Lawyer - Oakland CA
Susanne Stoffel - El Cerrito CA

Cetus Corporation - Emeryville CA

C12P 2100
C12N 1500
C12N 500

435 68

Expression vectors containing coding sequences under the control of SV40 early and RSV promoters are disclosed as useful in producing proteins in saccharomyces yeasts. Construction of such vectors, and their use in yeast transformations are described.

48309621, May 16, 1989

Feb 9, 1984

6/578122

David H. Gelfand - Oakland CA
Lawrence I. Greenfield - Albany CA
Frances C. Lawyer - Oakland CA

Cetus Corporation - Emeryville CA

C12P 2100
C12N 1500
C12N 120

435 68

Recombinant vectors which are effective in expressing DNA sequences encoding specific fragments of diphtheria toxins at high levels in recombinant host cells are disclosed. Both a fragment consisting of the enzymatically active A chain of diphtheria toxin and a fragment consisting of both the A portion and a B portion partial sequence are constructed by use of this recombinant vector.

54200296, May 30, 1995

Feb 3, 1993

7/971819

David H. Gelfand - Oakland CA
Frances C. Lawyer - Oakland CA
Susanne Stoffel - El Cerrito CA

Hoffmann-La Roche Inc. - Nutley NJ

C12N 1500
C12N 912

435194

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

56187114, Apr 8, 1997

Feb 6, 1995

8/384490

David H. Gelfand - Oakland CA
Frances C. Lawyer - Davis CA
Susanne Stoffel - El Cerrito CA

Hoffmann-La Roche Inc. - Nutley NJ

C12N 1554
C12N 912

435194

Recombinant DNA sequences encoding the DNA polymerase activity of Thermus thermophilus can be used to construct recombinant vectors and transformed host cells for production of the activity. T. thermophilus DNA polymerase is an. about. 94 kDa protein especially useful in the DNA amplification procedure known as the polymerase chain reaction.

53745531, Dec 20, 1994

Aug 13, 1990

7/567244

David H. Gelfand - Oakland CA
Frances C. Lawyer - Oakland CA

Hoffmann-La Roche Inc. - Nutley NJ

C12N 912
C12N 1500
C12N 120

4352523

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperaturecycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.