Forrest K Raffel

Columbus, OH

Research

Nationwide Childrens Hospital since 2009
Graduate Research Assistant
The Ohio State University since Jun 2008
Graduate Research Associate
Mayo Clinic Jun 2007 - Aug 2007
Summer Mentored Undergraduate Research Fellow
Occidental College Feb 2007 - Jun 2007
Teaching Assistant
Mayo Clinic Jun 2006 - Aug 2006
Payed Summer Internship

Occidental College 2004 - 2008
BA, Biology The Ohio State University College of Medicine 2008

Research
Data Analysis
Molecular Biology
Genetics
Laboratory
Teamwork
Pcr
Cell Culture
Teaching
Business Intelligence
Immunohistochemistry
Science
Western Blotting
Cell Biology
Elisa
Project Management
Hospitals
Biomedical Engineering
Lifesciences
Tissue Culture
Public Health
Protein Expression
Predictive Modeling
Business Requirements
Microsoft Office
Microsoft Excel
Microsoft Word

The Ohio State University Medical School and the Research Institute at Nationwide Children's Hospital
Columbus, OH
Jun 2009 to Dec 2013
Biomedical Sciences Graduate Program Dissertation Research
The Ohio State University Medical Center and the Research Institute at Nationwide Children's Hospital

Jul 2008 to Jun 2009
Biomedical Sciences Graduate Program Rotations
Occidental College
Los Angeles, CA
Sep 2007 to Dec 2007
Genetics Teaching Assistant
Transplantation Biology
Rochester, MN
Jun 2007 to Aug 2007
Summer Mentored Undergraduate Research Fellow
Occidental College
Los Angeles, CA
Jan 2007 to May 2007
Student Researcher
Interventional Radiology
Rochester, MN
Jun 2005 to Aug 2005
Research Assistant
Transplantation Biology
Rochester, MN
Jan 2005 to Jun 2005
Mentorship Student
Interventional Radiology
Rochester, MN
Jun 2004 to Aug 2004
Research Assistant

The Ohio State University College of Medicine
Columbus, OH
2008 to 2013
Doctor of Philosophy in Biomedical Sciences Graduate Program
Occidental College
Los Angeles, CA
2004 to 2008
Bachelor of Arts

Key Capabilities and Research Techniques Teamwork and Collaboration Working as part of a team comprising principle investigations, research scientists, research associates and interns Collaborations with internal and external partners Training and management of research associates and interns Communication Effective oral and poster presentations and local, national and international meetings Problem Solving Apply knowledge to critically and creatively solve scientific problems. Discuss and brainstorm with team members at weekly meetings to identify solutions and find innovative ways to carry out experiments Microbiology and Bacterial genetics Bacterial culture, storage, identification, BSL2 Cloning and bacterial genetic manipulation, gene deletion, complementation in trans, plasmid construction RNA isolation and quantitative real-time PCR DNA isolation and sequence analysis Biofilm formation and analysis Analysis of bacterial outer membrane properties Protein Protein isolation, fractionation, precipitation and purification Polyacrylamide gel electrophoresis, protein staining and Western blot Preparation of samples for Mass spectrometry Cytokine analysis by ELISA and immunoblot Antimicrobial peptide bactericidal killing assays, pulse-chase in vivo experiments and detection by Western blot Microscopy Preparation of bacterial, cell culture, and tissue samples for inverted microscopy, fluorescent microscopy, immuno-fluorescent microscopy, scanning electron microscopy and transmission electron microscopy Experience in visualization and image capture for inverted microscopy, fluorescent microscopy, immuno-fluorescent microscopy, scanning electron microscopy Mammalian cell culture Sterile technique, culture and propagation of primary and immortalized mammalian cells Isolation and cultivation of chinchilla middle ear epithelial cells and chinchilla nasopharyngeal epithelial cells Transwell model of epithelial cell growth to promote differentiation at an air-liquid interface Bacterial inoculation, Gentamicin protection assay, bacterial adherence assay Animal Modeling Experience with handling and restraint in murine and chinchilla animal models Administration of anesthesia Bacterial inoculation - intranasal, intraperitoneal, transbullar Humane euthanasia, dissection, collection of tissue samples, viable bacterial enumeration