Inventors:
Gerald R. Fink - Brookline MA
Joshua Trueheart - San Francisco CA
Elaine A. Elion - Somerville MA
Assignee:
Whitehead Institute for Biomedical Research - Cambridge MA
International Classification:
C12N 119
C12N 1531
C12N 1581
Abstract:
A yeast promoter inducible by the appropriate pheromone and a method of expressing a gene of interest in substantial quantities by placing it under the control of the inducible promoter. DNA encoding a protein of interest is fused or linked to a pheromone - inducible yeast promoter, such as the FUSI or the FUS2 promoter, and the fusion is inserted onto a high copy vector; the resulting product is introduced into wild type yeast cells. Stimulation of these yeast cells by the appropriate pheromone results in induction of transcription of the yeast promoter and expression of the protein of interest in substantial quantities.
