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Daniel L Bassoni

from Los Gatos, CA
Age ~54
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Also known as:
Daniel Louis Bassoni
Related to:
Karen B Huston, 51Julie Priest Hummel, 55Joel M Huston, 59Carol J Bassoni, 78Ronald A Bassoni, 82
Connected to:
Michelle A Bassos, 60Alban A Bassuet, 67Beth A Bast, 69Boris Bast, 50Crystal A Bast, 36Diane M Bast, 64Julian Bast, 36Oliver W Bast, 61Ray R Bast, 99Russell L Bast, 66

Daniel Bassoni Phones & Addresses

  • Los Gatos, CA
  • 335 3Rd St, Campbell, CA 95008 (408) 378-8744 (408) 866-7314
  • 335 N 3Rd St #3, Campbell, CA 95008 (408) 378-8744
  • 10115 Swan Oak Ln, Cupertino, CA 95014 (408) 252-3308
  • Placerville, CA
  • Davis, CA
  • Santa Clara, CA

Work

Company: Discoverx Jan 2006 Position: Group leader, assay development

Education

School / High School: University of California, Davis

Skills

Assay Development • Drug Discovery • High Throughput Screening • Cell Biology • Cell • Molecular Biology • Cell Culture • Gpcrs • Biochemistry • Laboratory Automation • In Vitro • Fluorescence • Immunology • Cell Signaling • Immunoassays • Genomics • Antibodies • Protein Chemistry • Enzyme Assays • Proteomics • Monoclonal Antibodies • Biomarkers • Signal Transduction • Flow Cytometry • Tissue Culture

Industries

Biotechnology

Resumes

Resumes

Daniel Bassoni Photo 1

Scientist, Assay Development

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Location:
Campbell, CA
Industry:
Biotechnology
Work:
DiscoveRx since Jan 2006
Group Leader, Assay Development
DiscoverX since Jan 2006
Research scientist
Vitra Biosciences Feb 2001 - Nov 2005
Research Associate
Education:
University of California, Davis
Skills:
Assay Development
Drug Discovery
High Throughput Screening
Cell Biology
Cell
Molecular Biology
Cell Culture
Gpcrs
Biochemistry
Laboratory Automation
In Vitro
Fluorescence
Immunology
Cell Signaling
Immunoassays
Genomics
Antibodies
Protein Chemistry
Enzyme Assays
Proteomics
Monoclonal Antibodies
Biomarkers
Signal Transduction
Flow Cytometry
Tissue Culture

Publications

Us Patents

Gpcr Arrestin Assays

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US Patent:
20100120063, May 13, 2010
Filed:
Oct 10, 2009
Appl. No.:
12/577175
Inventors:
Daniel Bassoni - Campbell CA, US
Keith R. Olson - Pleasanton CA, US
Thomas S. Wehrman - Mountain View CA, US
Assignee:
DISCOVERX CORPORATION - Fremont CA
International Classification:
G01N 33/53
US Classification:
435 72
Abstract:
Sensitive assays for candidate compounds affecting GPCR activity are provided using a cell containing fusion proteins comprising a first fusion protein comprising (a) a target GPCR fused to a small fragment of β-galactosidase through a linker comprising a phosphorylation site or (b) a GPCR or a protein of interest, where the GPCR and protein of interest form a complex and one of them is fused to the small fragment of β-galactosidase; and a second fusion protein comprising arrestin fused to a large fragment of β-galactosidase. In (a), the affinity of the small and large fragments is optimized based on the background to signal ratio and the absolute signal observed. The assay is performed using a β-galactosidase substrate that provides a detectable optical signal.

Monitoring Protein Trafficking Using Beta-Galactosidase Reporter Fragment Complementaton

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US Patent:
20120329075, Dec 27, 2012
Filed:
Jun 22, 2012
Appl. No.:
13/531230
Inventors:
Thomas S. Wehrman - Mountain View CA, US
Daniel Bassoni - Campbell CA, US
William Raab - San Francisco CA, US
Assignee:
DISCOVERX CORPORATION - Fremont CA
International Classification:
G01N 33/573
US Classification:
435 721, 435 78
Abstract:
Methods and materials are disclosed for use in an enzyme fragment complementation assay using complementary fragments of β-galactosidase to study the trafficking of proteins in a cell. Compounds that bind to a target peptide have been found to affect protein folding and therefore trafficking. β-Galactosidase fragments, an enzyme donor (ED) and an enzyme acceptor (EA), are fused to a target peptide and to an intracellular compartment protein, wherein the compartment is involved in intracellular trafficking. Contacting the cell with a compound that binds to the target peptide results in enhanced movement of the protein through the cellular trafficking pathway comprised of the endoplasmic reticulum, Golgi apparatus, the plasma membrane, endosomes, etc. Using this approach, compounds that bind to a target peptide and alter its ability to traffic through the normal cellular pathway can be readily detected.

Monitoring Protein Trafficking Using Beta-Galactosidase Reporter Fragment Complementation

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US Patent:
20140045194, Feb 13, 2014
Filed:
Oct 21, 2013
Appl. No.:
14/058822
Inventors:
Daniel Bassoni - Campbell CA, US
William Raab - Fremont CA, US
Assignee:
DiscoveRx Corporation - Fremont CA
International Classification:
C12Q 1/34
US Classification:
435 721
Abstract:
Methods and materials are disclosed for use in an enzyme fragment complementation assay using complementary fragments of β-galactosidase to study the trafficking of proteins in a cell. Compounds that bind to a target peptide have been found to affect protein folding and therefore trafficking. β-Galactosidase fragments, an enzyme donor (ED) and an enzyme acceptor (EA), are fused to a target peptide and to an intracellular compartment protein, wherein the compartment is involved in intracellular trafficking. Contacting the cell with a compound that binds to the target peptide results in enhanced movement of the protein through the cellular trafficking pathway comprised of the endoplasmic reticulum, Golgi apparatus, the plasma membrane, endosomes, etc. Using this approach, compounds that bind to a target peptide and alter its ability to traffic through the normal cellular pathway can be readily detected.
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Daniel L Bassoni from Los Gatos, CA, age ~54 Get Report