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Christian Sieracki Phones & Addresses

  • 232 Cross Point Rd, Edgecomb, ME 04556 (207) 882-7593 (207) 882-7808 (207) 882-8325
  • 263 Cross Point Rd, Edgecomb, ME 04556 (207) 882-7808
  • Palo Alto, CA
  • Santa Rosa, CA
  • Brookfield, VT
  • 232 Cross Point Rd, Edgecomb, ME 04556 (207) 882-7593

Work

Position: Educator

Education

Degree: Associate degree or higher

Publications

Us Patents

Oil-Immersion Enhanced Imaging Flow Cytometer

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US Patent:
7796256, Sep 14, 2010
Filed:
May 5, 2008
Appl. No.:
12/151262
Inventors:
Christian K. Sieracki - Edgecomb ME,
Kent A. Peterson - Yarmouth ME,
Assignee:
Fluid Imaging Technologies, Inc. - Yarmouth ME
International Classification:
G01N 21/00
US Classification:
356342, 356377, 356440, 356 72, 25033912
Abstract:
A flow chamber, imaging objective, condenser and imaging light source as part of an optical system includes an oil-immersion objective and high numerical aperture condenser matched to a rectangular flow chamber. The oil-immersion objective and flow chamber include a high index of refraction immersion oil so as to enhance the optical resolution and optical coupling therethrough. The imaging light source generates light which passes through the condenser, the flow chamber and then the objective before being focused onto an imaging camera. Fluorescence excitation passes through the objective to the flow chamber where the oil immersion configuration enhances the focus and collection of the light back through the objective.

System And Method For Monitoring Birefringent Particles In A Fluid

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US Patent:
8345239, Jan 1, 2013
Filed:
Aug 4, 2009
Appl. No.:
12/535536
Inventors:
Christian K. Sieracki - Edgecomb ME,
William H. Nelson - North Yarmouth ME,
Assignee:
Fluid Imaging Technologies, Inc. - Yarmouth NE
International Classification:
G01J 4/00
US Classification:
356365, 356336, 356337, 356369, 250225
Abstract:
An imaging system with an imaging mechanism which includes polarization analyzers, which may be crossed polarization analyzers, positioned to provide birefringence images of particles in the fluid passing through the flow chamber. Captured images are of high resolution and may be used in comparison to known images of a library of images. The system and related method enhance the accuracy and sensitivity of particle monitoring by utilizing birefringence imaging combined with particle analysis and the detection of each particle's characteristic features, such as crystalline features. The system includes a scatter detector used to trigger backlighting of the flow chamber and capture images of particles therein.

System And Method For Monitoring Blue-Green Algae In A Fluid

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US Patent:
20090283697, Nov 19, 2009
Filed:
May 16, 2008
Appl. No.:
12/122019
Inventors:
Christian K. Sieracki - Edgecomb ME,
Kent A. Peterson - Yarmouth ME,
William H. Nelson - Yarmouth ME,
Assignee:
FLUID IMAGING TECHNOLOGIES, INC. - Yarmouth ME
International Classification:
G21K 5/00
G01N 15/02
US Classification:
2504581, 738655
Abstract:
A particle detection system with a detection mechanism that includes detectors positioned to detect two different ranges of fluorescence produced by particles in the fluid in a flow chamber. Each of the detectors is arranged to generating a trigger signal whenever fluorescence is detected. The system and related method enhance the accuracy and sensitivity of blue-green algae monitoring by utilizing imaging flow cytometry combined with particle analysis and the measurement of the ratio of each particle's phycocyanin to chlorophyll b detected by using the two detectors configured for detection of two different fluorescence ranges, one associated with the phycocyanin and the other associated with the chlorophyll b. Optionally, captured images may be used in comparison to known images of a library of images.

System And Method For Sweeping Mirror Enhanced Imaging Flow Cytometry

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US Patent:
20120002029, Jan 5, 2012
Filed:
Jun 30, 2010
Appl. No.:
12/826850
Inventors:
Christian K. Sieracki - Edgecomb ME,
Scott N. Ellis - Topsham ME,
Kent A. Peterson - Yarmouth ME,
Assignee:
FLUID IMAGING TECHNOLOGIES, INC. - Yarmouth ME
International Classification:
H04N 7/18
US Classification:
348 79, 348 61, 348E07085
Abstract:
An imaging flow cytometry system and method which includes a flow chamber, tracking mirror, microscope and imaging optics, image capturing system, device to regulate fluid flow through the chamber, and backlighting generator. The tracking mirror moves at a rate matched to the particle velocity in the flow chamber so as to enhance the sample flow rates possible with the system while maintaining clear and accurate imaging. The backlighting generator passes through the flow chamber and the objective before being focused on the image capturing system. Detected scatter events initiate tracking by the mirror, resulting in imaging with reduced motion blur even at high rates of flow.

System And Method For Monitoring Birefringent Particles In A Fluid

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US Patent:
20120127298, May 24, 2012
Filed:
Jan 27, 2012
Appl. No.:
13/360260
Inventors:
Christian K. Sieracki - Edgecomb ME,
William H. Nelson - North Yarmouth ME,
Robert M. Grimm - Raymond ME,
Scott Ellis - Topsham ME,
Assignee:
FLUID IMAGING TECHNOLOGIES, INC. - Yarmouth ME
International Classification:
H04N 7/18
US Classification:
348 79, 348 61, 348E07085
Abstract:
An imaging system with an imaging mechanism which includes polarization analyzers, which may be crossed polarization analyzers, positioned to provide birefringence images of particles in the fluid passing through the flow chamber. Captured images are of high resolution and may be used in comparison to known images of a library of images. The system and related method enhance the accuracy and sensitivity of particle monitoring by utilizing birefringence imaging combined with particle analysis and the detection of each particle's characteristic features, such as crystalline features. The system includes a scatter detector used to trigger backlighting of the flow chamber and capture images of particles therein.

Fluid Submersible Sensing Device

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US Patent:
20120176616, Jul 12, 2012
Filed:
Jun 17, 2010
Appl. No.:
13/379964
Inventors:
Carlton D. Hunt - Bridgewater MA,
Derek M. Michelin - Plymouth MA,
Michael P. Neal - Powell OH,
Christian K. Sieracki - Edgecomb ME,
J. Caleb Chitwood - Canal Winchester OH,
Douglas B. Pape - Upper Arlington OH,
David A. Fingerhuth - Marysville OH,
International Classification:
G01N 21/00
US Classification:
356338
Abstract:
A fluid submersible sensing device is provided comprising a fluid-tight housing defining an internal chamber and including window structure; sensing structure provided in the internal chamber; light providing apparatus in the internal chamber emitting light capable of passing through the window structure so as to exit the housing; and a sample-providing structure coupled to the housing and located outside of the housing internal chamber comprising a passage through which a fluid flows. The passage may have a longitudinal axis substantially parallel with a flow path through the passage and a cross sectional area substantially transverse to the longitudinal axis. The light from the housing exits the housing, passes through the sample-providing structure including the passage and re-enters the housing toward the sensing structure.

System And Method For Total Internal Reflection Enhanced Imaging Flow Cytometry

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US Patent:
20130315447, Nov 28, 2013
Filed:
May 25, 2012
Appl. No.:
13/480999
Inventors:
Christian K. Sieracki - Edgecomb ME,
Kent A. Peterson - Yarmouth ME,
Matthew A. Duplisea - Gorham ME,
Corie F. Drake - Portland ME,
Assignee:
FLUID IMAGING TECHNOLOGIES, INC. - Yarmouth ME
International Classification:
G06K 9/00
US Classification:
382107
Abstract:
An imaging flow cytometry system and method which includes a flow chamber, fluorescence analysis and imaging optics, image capturing system, device to regulate fluid flow through the chamber, and backlighting generator. The flow cell is configured so as to enhance the fluorescence signal collection by the system with total internal reflections. The fluorescence collection optics are configured to enhance the collection of the fluorescence from the side of the flow cell and concentrate it on light detectors.

Device And Method For Studying Particles In A Fluid

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US Patent:
6115119, Sep 5, 2000
Filed:
Oct 21, 1998
Appl. No.:
9/176678
Inventors:
Christian K. Sieracki - Edgecomb ME
Michael E. Sieracki - Edgecomb ME
Charles S. Yentsch - West Boothbay Harbor ME
Assignee:
Bigelow Laboratory for Ocean Science - West Boothbay Harbor ME
International Classification:
G01N 2100
US Classification:
356337
Abstract:
The present invention provides a device for studying particles in a fluid that advantageously allows particles having a broad range of particle size, e. g. , in the 3-1000. mu. m range, to be readily imaged and counted.
Christian K Sieracki from Edgecomb, ME, age ~63 Get Report