Work:
Howard Hughes Medical Institute/Children's Hospital Boston
Jan 2008 to 2000
Research associate
City of Hope Graduate School of Biological Sciences
Jan 2002 to Jan 2008
Graduate researcher
Education:
City of Hope Graduate School of Biological Sciences
Duarte, CA
Jun 2008
Ph.D. in Developmental Neurobiology
University of Science and Technology of China
Jun 2002
B.S. in Biology
Skills:
Cell biology: bacterial cell culture, mammalian cell culture (cell line and primary neuron), cell-based assays (transfection, reporter assays, pharmacological assays), immunocytochemistry Microscopy: light microscopy, fluorescent microscopy, confocal microscopy, 2-photon microscopy, image analysis (imageJ, ImagePro, NeuroMath) Molecular biology: DNA and RNA extraction, PCR, RT-PCR, cloning and subcloning, plasmid construction, southern blot Biochemistry: protein assays, co-immunoprecipitation, SDS-PAGE, western blot, protein purification Histology and histopathology: RNA in situ hybridization, whole mount immunohistochemistry, section immunohistochemistry (paraffin section, vibratome section, cryo-section) Animal-related techniques: mouse surgery, in utero electroporation, intraperitoneal and subcutaneous injection, mouse behavior testing, colony management