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Billy Louis Kolen

from Marshville, NC
Age ~81
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Also known as:
Billy L Kolen, Billy K Kolen, William L Kolen, William K Kolen, Bill L Kolen, Bill K Kolen
Phone and address:
7022 Rebel Rd, Olive Branch, NC 28103
(704) 624-9108
Related to:
Quortney KolenIsaiah ColesRochelle K Kymberle
Connected to:
Patti L Kolen, 57Suzanne S Kolen, 60David P Kolena, 73Laura C Kolena, 39Hendrik Kolenbrander, 71Joseph J Kolencik, 44Catherine M Kolenda, 97Christina L Kolenda, 47Frank Kolenda, 78James C Kolenda, 64

Billy Kolen Phones & Addresses

  • 7022 Rebel Rd, Marshville, NC 28103 (704) 624-9108
  • 2121 Commerce Dr #101, Monroe, NC 28110
  • Bowie, MD
  • 3108 Foxglove Way, Upper Marlboro, MD 20774
  • Springdale, MD
  • Temple Hills, MD
  • Glenarden, MD
  • Union, NC
  • Clinton, MD
  • 7022 Rebel Rd, Marshville, NC 28103

Publications

Us Patents

Method For The Production Of Pure Virally Inactivated Butyrylcholinesterase

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US Patent:
7365173, Apr 29, 2008
Filed:
Feb 4, 2002
Appl. No.:
10/061233
Inventors:
Annemarie H. Ralston - Bethesda MD, US
Billy L. Kolen - Springdale MD, US
David John Hammond - Laytonsvile MD, US
Assignee:
American National Red Cross - Washington DC
International Classification:
C07K 1/14
C07K 1/18
C07K 1/22
US Classification:
530412, 530416, 530422
Abstract:
The present invention provides a method for purifying butyrylcholinesterase from various biological fluids. Biological fluids include, e. g. , blood, blood fractions, plasma, and bioreactor broths, and other such mixtures containing butyrylcholinesterase. In one embodiment, the invention provides a method for the production of purified, virally inactivated butyrylcholinesterase by contacting a biological fluid containing butyrylcholinesterase with a cationic exchange chromatography material, with an affinity chromatography material, and treating the fluid with solvent detergent. The resulting purified butyrylcholinesterase can also be subjected to a pasteurization step, and formulated in a sodium chloride/sodium phosphate solution for storage or lyophilization.

Method For The Production Of Pure Virally Inactivated Butyrylcholinesterase

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US Patent:
20090093038, Apr 9, 2009
Filed:
Mar 13, 2008
Appl. No.:
12/076107
Inventors:
Annemarie H. Ralston - Bethesda MD, US
Billy L. Kolen - Springdale MD, US
David John Hammond - Laytonsville MD, US
Assignee:
American National Red Cross - Washington DC
International Classification:
C12N 9/99
C12N 9/14
US Classification:
435184, 435195
Abstract:
The present invention provides a method for purifying butyrylcholinesterase from various biological fluids. Biological fluids include, e.g., blood, blood fractions, plasma, and bioreactor broths, and other such mixtures containing butyrylcholinesterase. In one embodiment, the invention provides a method for the production of purified, virally inactivated butyrylcholinesterase by contacting a biological fluid containing butyrylcholinesterase with a cationic exchange chromatography material, with an affinity chromatography material, and treating the fluid with solvent detergent. The resulting purified butyrylcholinesterase can also be subjected to a pasteurization step, and formulated in a sodium chloride/sodium phosphate solution for storage or lyophilization.
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Billy Louis Kolen from Marshville, NC, age ~81 Get Report