Inventors:
A. Robert Neurath - New York NY
Alfred M. Prince - Stamford CT
Arnold Lippin - Brooklyn NY
Assignee:
The Community Blood Council of Greater New York, Inc. - New York NY
International Classification:
A61K 3912
C07G 7028
C12K 700
Abstract:
After a polyethylene glycol purification procedure, virus particles, viral components and virus associated particles having concanavalin A binding sites are further purified by subjecting such particles or components to affinity chromatography utilizing insoluble concanavalin A as a chromatography adsorbent and an eluant capable of interacting with concanavalin A to thereby inhibit the binding of the virus particles or components to the insoluble concanavalin A. Specifically, the insoluble concanavalin A may be concanavalin A linked to agarose beads and the eluant may be methyl-. alpha. -D-mannopyranoside. Specifically, these procedures may be utilized to purify hepatitis B antigen (HB Ag) particles.