Apostolos G Doukas

6428532, Aug 6, 2002

Dec 14, 1999

09/460860

Apostolos Doukas - Belmont MA
Shun Lee - Boston MA

The General Hospital Corporation - Boston MA

A61B 1818

606 9, 606 10, 606 11, 606 33, 359245, 359325, 359326, 359349, 372 21

A method and apparatus for treating tissue directs first and second wavelengths of energy, e. g. , laser or ultrasonic signal energy, into an overlap region of tissue such that the first and second wavelengths mix in situ to form a third wavelength. The third wavelength effectively couples energy into the tissue to treat tissue in said overlap region, producing local heating or cavitation. Wavelengths may be selected such that overlying tissue is unharmed, and the two wavelengths may be applied along different paths and cross in the overlap region. Absorbers or dyes may be used to tailor absorbance of the third wavelength. When using ultrasonic energy, signals of several megahertz may be so mixed to provide a lower frequency treatment signal such as a 200 kHz treatment signal or a subharmonic. Laser signals may be mixed to form a treatment signal in the infrared.

6562004, May 13, 2003

Jun 5, 2000

09/587333

Apostolos Doukas - Belmont MA
Shun Kwan Lee - Boston MA
Thomas J. Flotte - Boston MA

The Massachusetts General Hospital - Boston MA

A61M 3700

604145, 604140

A device for transdermal delivery of a composition includes a housing partitioned into a delivery chamber arranged to contain the composition and a detonation chamber mechanically coupled to the delivery chamber. The detonation chamber is intended to contain an energetic material producing an impulse transient in response to a detonating stimulus. The housing is coupled to an energy coupling element, or detonator, for communicating energy provided by an external energy source into the detonating stimulus in the detonation chamber. The detonator can detonate the energetic material by causing an electrical discharge or spark within the detonation chamber. The detonator can be a piezoelectric film in electrical communication with the energetic material.

6689094, Feb 10, 2004

Jun 25, 2001

09/891096

Nikiforos Kollias - Watertown MA
Apostolos G. Doukas - Belmont MA
Thomas J. Flotte - Boston MA
Daniel J. McAuliffe - Boxford MA
Shun Lee - Boston MA

The General Hospital Corporation - Boston MA

A61M 3100

604 69

A method for delivering compounds through epithelial cell layers using impulse transients is described. The method involves applying a compound to, e. g. , the stratum corneum, of a patient and then inducing impulse transients to create transient increases in the permeability of epithelial tissue, thereby facilitating delivery of the compound across the epithelial cell layer.

7332200, Feb 19, 2008

May 12, 2000

10/019837

Nikolaos S. Soukos - Revere MA, US
Shun Lee - Arlington VA, US
Apostolos G. Doukas - Belmont MA, US

The General Hospital Corporation - Boston MA

B05D 3/06

427554

Methods for permeabilizing biofilms using stress waves are described. The methods involve applying one or more stress waves to a biofilm, e. g. , on a surface of a device or food item, or on a tissue surface in a patient, and then inducing stress waves to create transient increases in the permeability of the biofilm. The increased permeability facilitates delivery of compounds, such as antimicrobial or therapeutic agents into and through the biofilm.

20030176836, Sep 18, 2003

Apr 16, 2003

10/414928

Apostolos Doukas - Belmont MA, US
Shun Lee - Boston MA, US
Thomas Flotte - Boston MA, US

Massachusetts General Hospital,

A61M031/00

604/093010, 604/069000

A device for transdermal delivery of a composition includes a housing partitioned into a delivery chamber arranged to contain the composition and a detonation chamber mechanically coupled to the delivery chamber. The detonation chamber is intended to contain an energetic material producing an impulse transient in response to a detonating stimulus. The housing is coupled to an energy coupling element, or detonator, for communicating energy provided by an external energy source into the detonating stimulus in the detonation chamber. The detonator can detonate the energetic material by causing an electrical discharge or spark within the detonation chamber. The detonator can be a piezoelectric film in electrical communication with the energetic material.

20040158192, Aug 12, 2004

Feb 5, 2004

10/772668

Nikiforos Kollias - Watertown MA, US
Apostolos Doukas - Belmont MA, US
Thomas Flotte - Boston MA, US
Daniel McAuliffe - Boxford MA, US
Shun Lee - Boston MA, US

The General Hospital Corporation, a Massachusetts corporation

A61N001/30

604/020000

A method for delivering compounds through epithelial cell layers using impulse transients is described. The method involves applying a compound to, e.g., the stratum corneum, of a patient and then inducing impulse transients to create transient increases in the permeability of epithelial tissue, thereby facilitating delivery of the compound across the epithelial cell layer.

20060075514, Apr 6, 2006

May 20, 2005

11/134565

Thomas Flotte - Boston MA, US
Apostolos Doukas - Belmont MA, US

C12N 15/01
A01N 1/02

800021000, 435446000, 435284100

A method for temporarily permeabilizing a nuclear membrane to allow a molecule to enter a nucleus of a cell includes exposing the cell to a fluid medium containing the molecule; and causing, in the fluid medium an impulse having a peak pressure sufficient to permeabilize the nuclear membrane.

20090259167, Oct 15, 2009

Mar 23, 2009

12/409150

Fernanda H. Sakamoto - Boston MA, US
R. R. Anderson - Boston MA, US
Zeina Tannous - Boston MA, US
William A. Farinelli - Danvers MA, US
Apostolos G. Doukas - Belmont MA, US

THE GENERAL HOSPITAL CORPORATION - Boston MA

A61N 5/06
A61K 31/197
A61P 35/00

604 21, 604 20, 514561

The invention provides methods and compositions for treating a tissue disorder in a subject by parenterally administering a solution of aminolevulinic acid (ALA) or a derivative thereof that is not greater than 1.0 percent by weight into a local subcutaneous or dermal region of the subject; and administering high fluence light to said bodily area to produce a phototoxic species in said local region, thereby treating a tissue disorder in the subject.