Inventors:
Anthony P. Shuber - Milford MA, 01757
Stanley N. Lapidus - Bedford NH, 03110
International Classification:
C12P 1934
US Classification:
435 912, 435 6, 435 911, 436 94, 536 231, 536 243, 536 2433
Abstract:
Methods are provided for selective nucleic acid sequence detection in single base primer extension reactions of high sensitivity. These methods are useful for detecting small amounts of mutant nucleic acid in a heterogeneous biological sample. These methods are particularly useful for identifying individuals with gene mutations indicative of early colorectal cancer.