Andrei L Gindilis

8232109, Jul 31, 2012

Jul 31, 2007

11/888491

John W. Hartzell - Camas WA, US
Pooran Chandra Joshi - Vancouver WA, US
Paul J. Schuele - Washougal WA, US
Andrei Gindilis - Vancouver WA, US

Sharp Laboratories of America, Inc. - Camas WA

G01N 21/00

436164, 436 2, 436805, 385 15, 385 17, 4352891, 4352871, 4352885, 422 681, 422 8205

A method of performing a fluid-material assay employing a device including at least one active pixel having a sensor with an assay site functionalized for selected fluid-assay material. The method includes exposing the pixel's sensor assay site to such material, and in conjunction with such exposing, and employing the active nature of the pixel, remotely requesting from the pixel's sensor assay site an assay-result output report. The method further includes, in relation to the employing step, creating, relative to the sensor's assay site in the at least one pixel, a predetermined, pixel-specific electromagnetic field environment.

8234905, Aug 7, 2012

Aug 4, 2008

12/185253

Andrei L. Gindilis - Vancouver WA, US

Sharp Laboratories of America, Inc. - Camas WA

G01N 33/53
G01N 37/00

73 101

A system and method are provided for selectively functionalizing a transducer microarray. The method provides a microarray including a field of transducers exposed to a shared local environment. A difference in the pH associated with the transducers is created. As a result, functional molecules are selectively bound to transducers in response to the pH associated with the transducers. In one aspect, the micro-array also provides a field of transducer pH-generating electrodes, one pH-generating electrode for each transducer, and a counter electrode. The difference in pH associated with the transducers is created by selectively applying a voltage potential between pH-generating electrodes and the counter electrode, to create a difference of pH in regions adjacent to the transducers.

20010053529, Dec 20, 2001

Sep 8, 1998

09/148900

ANDREI L. GINDILIS - DOVER NJ, US

G01N033/53

435/007100

The present invention relates to assays that employ an enzyme label or tag that acts on a substrate by obtaining electrons from an electrode (electrocatalysis) and an apparatus for use in such assays. In particular the present invention provides an assay and apparatus for multiple or continuous monitoring of the amount of analyte in a sample or sample source without requiring regeneration of the measuring electrode or its associated reagents.

20020055127, May 9, 2002

Dec 17, 2001

10/023797

Andrei Gindilis - Mukilteo WA, US

G01N033/53
G01N033/542
G01F001/64

435/007900, 205/777500

The present invention relates to assays that employ an enzyme label or tag that acts on a substrate by obtaining electrons from an electrode (electrocatalysis) and an apparatus for use in such assays. In particular the present invention provides an assay and apparatus for multiple or continuous monitoring of the amount of analyte in a sample or sample source without requiring regeneration of the measuring electrode or its associated reagents.

20060102471, May 18, 2006

Nov 18, 2004

10/992252

Karl Maurer - Everett WA, US
John Cooper - Seattle WA, US
Michael Strathmann - Seattle WA, US
Andrei Gindilis - Mukilteo WA, US

B23H 3/04
B08B 6/00
C23F 1/00

204290010, 134001100, 216067000

There is disclosed an electrode array device having an adsorbed porous reaction layer for improved synthesis quality. The array comprises a plurality of electrodes on a substrate, wherein the electrodes are electronically connected to a computer control system. The array has an adsorbed porous reaction layer on the plurality of electrodes, wherein the adsorbed porous reaction layer comprises a chemical species having at least one hydroxyl group. In the preferred embodiment, the reaction layer is sucrose. A method for preparing an electrode array for improved synthesis quality is disclosed. The method comprises a cleaning method and a method of attachment of a reaction layer. The cleaning method comprises a plasma cleaning method and a chemical cleaning method. The reaction layer is attached after cleaning by exposing the microarray to a solution containing the chemical species having at least one hydroxyl group.

20070037169, Feb 15, 2007

Aug 9, 2005

11/161607

John Cooper - Seattle WA, US
Andrei Gindilis - Mukilteo WA, US

COMBIMATRIX CORPORATION - Mukilteo WA

C12Q 1/68

435006000, 204450000

The present invention provides a method for selective dehybridization by electrochemically-generated (ECG) reagent on an electrode microarray. The ECG reagent is generated by activation of selected electrodes. Activation alters pH in the vicinity of only the selected electrodes. In one embodiment, the increase or decrease in pH is sufficient to cause dehybridization of an oligonucleotide duplex at the selected electrodes. In another embodiment, the increase or decrease in pH is sufficient to prevent chemical dehybridization at the selected electrodes. The dehybridized single stranded target oligonucleotide may be recovered and amplified by PCR.

20100069253, Mar 18, 2010

Sep 12, 2008

12/210062

Andrei L. Gindilis - Vancouver WA, US

C40B 30/04
C40B 40/08

506 9, 506 17

An impedance spectroscopy system and method are provided for quantitatively measuring DNA. The method provides a transducer having electrode surfaces exposed to a shared local environment. The electrode surfaces are functionalized with an oligonucleotide to interact with a predetermined DNA target. A DNA sample solution is introduced into the local environment. The solution includes nucleotides, polymerase enzyme, and primers. The DNA sample is thermocycled to promote a first DNA target polymerase chain reaction (PCR). Then, capacitance is measured between a pair of transducer electrodes, and in response to measuring the capacitance, a determination is made of the presence of first DNA amplicons in the DNA sample. Typically, a number of thermocycles are performed and capacitance measurements are made after each cycle, so that an amplicon growth rate can be determined.

20110046350, Feb 24, 2011

Aug 24, 2009

12/546227

Andrei L. Gindilis - Vancouver WA, US
Kevin Robert Schwarzkopf - Camas WA, US

C07K 14/00
C07C 55/02
C07H 21/04

530350, 562590, 536 231

Embodiments of the present invention are directed to reusable, length-adjustable nanoscale tethers that can be incorporated into a variety of different sensors and other electrical, electro-mechanical, electro-optical-mechanical, and optical-mechanical devices. An optionally reusable, length-adjustable nanoscale tether that represents one embodiment of the present invention comprises a binding-structure component, having a substrate-anchor subcomponent and a binding-adapter binding domain, and a binding adaptor that binds to the binding-adapter binding domain and that has a target-binding subcomponent that binds to a target molecule, target particle, or other target entity. The binding-adapter binding domain can be positioned at different distances from the substrate anchor within the binding-structure component so that the distance between a bound target and a sensor substrate can be precisely controlled.