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Andreas G Seyfang

from Tampa, FL
Age ~64

Andreas Seyfang Phones & Addresses

  • 6037 Catlin Dr, Tampa, FL 33647 (706) 855-1147
  • 14406 Audubon Ave, Tampa, FL 33613
  • s
  • 6001 Laketree Ln #F, Tampa, FL 33617
  • Temple Terrace, FL
  • 4412 Peregrine Ct, Martinez, GA 30907
  • 2314 Spring House Ln, Augusta, GA 30907
  • Portland, OR
  • Griffin, GA

Work

Company: Medical college of georgia Oct 1999 to Jun 2005 Position: Assistant professor

Education

Degree: Doctorates, Doctor of Philosophy School / High School: University of Tübingen 1989 to 1993 Specialities: Biochemistry

Skills

Biochemistry • Molecular Biology • Microbiology • Cell Biology • Western Blotting • Cell Culture • Pcr • Teaching • Mentoring • Confocal Microscopy • Microscopy • Statistics • Immunohistochemistry • Parasitology • Polymerase Chain Reaction

Languages

German • English • French • Spanish

Interests

Pathogenic Fungi • Leishmania • Gene Expression Profiling • Candida Albicans • Protein Biochemistry • Cryptococcus Neoformans • Structure Function Analysis

Industries

Research

Resumes

Resumes

Andreas Seyfang Photo 1

Professor

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Location:
Tampa, FL
Industry:
Research
Work:
Medical College of Georgia Oct 1999 - Jun 2005
Assistant Professor

Ohsu | Oregon Health & Science University Aug 1995 - Sep 1999
Postdoctoral Fellow

Université De Bordeaux Sep 1993 - Aug 1995
Postdoctoral Fellow

University of South Florida Sep 1993 - Aug 1995
Professor
Education:
University of Tübingen 1989 - 1993
Doctorates, Doctor of Philosophy, Biochemistry
University of Tübingen 1986 - 1989
Master of Science, Masters, Zoology
Duke University 1984 - 1985
University of Tübingen 1981 - 1983
Bachelors, Bachelor of Science, Biology
University of Tübingen
Master of Science, Doctorates, Masters, Doctor of Philosophy
Skills:
Biochemistry
Molecular Biology
Microbiology
Cell Biology
Western Blotting
Cell Culture
Pcr
Teaching
Mentoring
Confocal Microscopy
Microscopy
Statistics
Immunohistochemistry
Parasitology
Polymerase Chain Reaction
Interests:
Pathogenic Fungi
Leishmania
Gene Expression Profiling
Candida Albicans
Protein Biochemistry
Cryptococcus Neoformans
Structure Function Analysis
Languages:
German
English
French
Spanish

Publications

Us Patents

Method For Multiple Site-Directed Mutagenesis

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US Patent:
6878531, Apr 12, 2005
Filed:
Jan 29, 2004
Appl. No.:
10/766190
Inventors:
Andreas Seyfang - Augusta GA, US
Assignee:
Medical College of Georgia Research Institute - Augusta GA
International Classification:
C12P019/34
US Classification:
435 912, 435 911
Abstract:
The present invention provides a simple and rapid method for site-directed mutagenesis of more than, for example, 10 sites simultaneously with up to 100% efficiency. The method uses two terminal tailed primers, specific for each end of the gene (or DNA sequence) to be mutated, with a unique nucleotide tail each that are simultaneously annealed to template DNA together with a set of mutagenic primers in-between. Following synthesis of the mutant strand by primer extension and ligation with, for example, T4 DNA polymerase and ligase, the unique mutant strand-specific tails of the terminal primers are used as anchors to specifically amplify the mutant strand by high-fidelity polymerase chain reaction. Furthermore, specific restriction endonuclease sites in the two anchor primer tails may be used for convenient subcloning of the PCR product in any desired cloning or expression vector (for subsequent sequencing or expression and functional studies of the mutated gene).

Transfection Vector For Pathogenic Amoebae And Uses Thereof

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US Patent:
20180245086, Aug 30, 2018
Filed:
Feb 12, 2018
Appl. No.:
15/894746
Inventors:
Andreas Seyfang - Tampa FL, US
Christopher L. Massengill - Odessa FL, US
Sara R. Sievers - San Carlos CA, US
Assignee:
University of South Florida - Tampa FL
International Classification:
C12N 15/65
C12N 15/79
A61K 39/002
C12N 15/87
C12N 13/00
C12N 1/10
Abstract:
Disclosed herein are expression vectors suitable for transfection in amoebas. The vectors may include a promoter from a protein-encoding gene from an amoeba, a selection marker, and a polynucleotide sequence encoding a polypeptide of interest, operably linked to the promoter. The promoter may be from the ACT1 gene from
Andreas G Seyfang from Tampa, FL, age ~64 Get Report