Stephen L Hughes

6492166, Dec 10, 2002

Jan 19, 2001

09/744022

Andrea L. Ferris - Frederick MD
Stephen H. Hughes - Smithsburg MD

The United States of America as represented by the Secretary of the Department of Health and Human Services - Washington DC

C12N 1574

4353201, 435455, 435456, 536 231

An Avian-derived retroviral vector from which the Constitutive Transport Element (CTE) has been removed and replaced with a genetic fragment from a mammalian virus in order to produce a vector that is replication competent in at least one non-native cell type without the need for a âhelperâ virus, and that is unlikely to recombine with endogenous mammalian viruses, making it both safe and simple to use in research and gene-therapy applications.

6794188, Sep 21, 2004

Sep 24, 2001

09/961933

Eugene Barsov - Frederick MD
Stephen H. Hughes - Smithsburg MD

The United States of America as represented by the Department of Health and Human Services - Washington DC

C12N 1586

435456, 435325, 4353201, 435455, 514 44, 424 931, 424 932, 800 13, 800 18

Recombinant avian sarcoma leukosis virus (ASLV)-derived retrovirus vectors having an expanded host range are described. The host range is expanded by the replacement of the ASLV envelope gene by an envelope gene from a virus capable of infecting both mammalian and avian cells. The resulting recombinant ASLV-derived retroviral vectors can replicate efficiently in avian cells, infect both avian and mammalian cells in high titer, and are replication-defective in mammalian cells. Thus, they are quite safe and advantageous for use in gene therapy and vaccines.

8513214, Aug 20, 2013

Nov 10, 2008

12/741873

Maqbool A. Siddiqui - Silver Spring MD, US
Victor E. Marquez - Montgomery Village MD, US
Stephen H. Hughes - Smithsburg MD, US
Paul L. Boyer - Greencastle PA, US

The United States of America as Represented by the Secretary of the Dept. of Health and Human Services - Washington DC

A01N 43/04
A61K 31/70
C07H 19/16
C07H 19/167
C07H 19/173
C12Q 1/70

514 45, 514 46, 435 5, 536 2721, 536 276

The invention provides for novel 2-Deoxyadenosine compounds, which can treat HIV infection at low cytotoxicity values. Substitution at the 4′-position provided compounds which demonstrated low cytotoxicity values in an ATP-based cytotoxicity assay.

60965347, Aug 1, 2000

Nov 28, 1997

8/875509

Eugene Barsov - Frederick MD
Stephen H. Hughes - Smithsburg MD

The United States of America as represented by the Department of Health
and Human Services - Washington DC

C12N 1500
C12N 700
C12N 510

4353201

Recombinant avian sarcoma leukosis virus (ASLV)-derived retrovirus vectors having an expanded host range are described. The host range is expanded by the replacement of the ASLV envelope gene by an envelope gene from a virus capable of infecting both mammalian and avian cells. The resulting recombinant ASLV-derived retroviral vectors can replicate efficiently in avian cells, infect both avian and mammalian cells in high titer, and are replication-defective in mammalian cells. Thus, they are quite safe and advantageous for use in gene therapy and vaccines.

20140273137, Sep 18, 2014

Mar 15, 2013

13/837654

The Unites Stated of America, as represented by the Secretary of Agriculture - , US
Dwayne R. Roach - Laurel MD, US
Piyum A. Khatibi - Dunlap IL, US
Kenneth M. Bischoff - Morton IL, US
Stephen R. Hughes - Peoria IL, US

The United States of America, as represented by the Secretary of Agriculture - Washington DC

C12P 7/10
C12P 7/06

435165, 435161, 435163

Ethanol losses due to bacterial contamination in fermentation cultures weakens the economics of biofuel production. species are the predominant contaminant. Bacteriophage lytic enzymes are peptidoglycan hydrolases which degrade Gram positive cell walls when exposed externally and are a novel source of antimicrobials. The streptococcal phage λSA2 endolysin construct demonstrated strong lytic activity towards 17 of 22 strains of lactobacilli, staphylococci or streptococci maintaining optimal specific activity under fermentation conditions toward substrates. bacteriophage endolysin constructs LysA, LysA2 and LysgaY showed exolytic activity towards 60% of the lactobacilli tested including four isolates from fuel ethanol fermentations. Presence of ethanol (≦5%) did not affect lytic activity. Lysins were able to reduce both and contaminants in mock fermentations of corn fiber hydrolysates. Recombinant LysA and λSa2 expressed in the yeast are functional; LysA was shown to reduce lactobacilli in experimentally infected fermentations.