Mark A Cooper

6339065, Jan 15, 2002

Jan 30, 1996

08/594299

Mark J. Cooper - Solon OH

Case Western Reserve University - Cleveland OH

A61K 3170

514 44, 435 691, 435455

Episomal plasmids containing a papovavirus origin of replication and a papovavirus large T antigen mutant form are shown to replicate episomally in human cells, and yield levels of gene expression proportional to their episomal copy number. In conjunction with liposomal or receptor-mediated delivery systems, papovavirus-derived episomal plasmids provide an alternative vector for gene therapy, particularly when utilizing strategies requiring high levels of gene expression.

6506890, Jan 14, 2003

Nov 28, 2000

09/722340

Mark J. Cooper - Solon OH, 44139
Tomasz H. Kowalczyk - University Heights OH, 44118
Murali Krishna Pasumarthy - Willoughby Hills OH, 44092

C07H 2102

536 231, 536 221, 435 6, 435 71, 435 911, 435 912

An automated nucleic acid compaction device for analyzing and compacting a nucleic acid complex into unimolecular nucleic acid particles. Broadly, the device includes a container support and agitation system; a measuring and testing system; and a dispensing system; all controlled by a control system. The control system controls the support and agitation system and the dispensing system based either on a predetermined formulation or by analysis of feedback data provided by the measuring and testing system. In a preferred embodiment, the device is a real-time measuring and mixing instrument operating in a closed loop system. The preferred embodiment also comprises a monitoring system including a submersible probe which is positioned in the batch solution to provide data to a controller. Once a desired level of nucleic acid compaction is reached, as detected by the monitoring system, the controller stops the dispensing and mixing agitating systems.

8017577, Sep 13, 2011

Sep 8, 2003

10/656192

Mark J. Cooper - Moreland Hills OH, US
Murali K. Pasumarthy - Twinsburg OH, US
Tomasz H. Kowalczyk - University Heights OH, US
Maureen Costello - Beachwood OH, US

Copernicus Therapeutics, Inc. - Cleveland OH

A61K 48/00

514 8, 514 44

Counterions of polycations used to compact nucleic acids profoundly affect shape and stability of particles formed. Shape is associated with differential serum nuclease resistance and colloidal stability. A surrogate for determining such properties that is easy to measure is the turbidity parameter. Shape also affects the suitability and efficacy of compacted nucleic acid complexes for transfecting cells by various routes into a mammalian body. Moreover, counterions such as acetate can protect compacted nucleic acid complexes from adverse effects of lyophilization.

8389238, Mar 5, 2013

Sep 12, 2008

12/676426

Mark J. Cooper - Moreland Hills OH, US
Linas Padegimas - Mayfield Heights OH, US

Copernicus Therapeutics, Inc. - Cleveland OH

C12Q 1/68
C12P 19/34
C12P 21/06
C12N 15/00
C07H 21/02

435 691, 435 6, 435 701, 435 911, 4353201, 536 231, 536 241, 536 242

Efficient and prolonged hCFTR expression is one of the major obstacles for cystic fibrosis lung therapy. hCFTR mRNA expression levels depend on eukaryotic expression cassette components, prokaryotic backbone elements, and the gene transfer method may also influence transcriptional silencing mechanisms. A codon-optimized and CpG-reduced human CFTR gene (CO-CFTR) was made. Various vector modifications were tested to facilitate extended duration of CO-CFTR expression. Insertion of an extended 3′BGH transcribed sequence (712 bp) in an inverted orientation produced prolonged expression of CO-CFTR expression at biologically relevant levels. Further studies revealed that prolonged CO-CFTR expression is dependant on the orientation of the extended BGH 3′ BGH transcribed sequence and its transcription, is not specific to the UbC promoter, and is less dependent on other vector backbone elements.

20020031803, Mar 14, 2002

Aug 24, 2001

09/935368

Mark Cooper - Moreland Hills OH, US

C12P021/02
C07K014/705
C07H021/04

435/069100, 435/325000, 435/320100, 536/023500, 530/350000

Novel methods are described for expression of biologically functional therapeutic and other proteins without host cell toxicity. The methods take advantage of the surprising replication activating ability of the 107/402-T antigen. The invention also provides fusion proteins, expression vectors, and mammalian cells, for practicing the methods.

20020042388, Apr 11, 2002

May 31, 2001

09/867693

Mark Cooper - Moreland Hills OH, US
Murali Pasumarthy - Twinsburg OH, US
Tomasz Kowalczyk - University Heights OH, US
Maureen Costello - Margate City NJ, US

A61K048/00
C12Q001/68

514/044000, 435/006000

Counterions of polycations used to compact nucleic acids profoundly affect shape and stability of particles formed. Shape is associated with differential serum nuclease resistance and colloidal stability. A surrogate for determining such properties that is easy to measure is the turbidity parameter. Shape also affects the suitability and efficacy of compacted nucleic acid complexes for transfecting cells by various routes into a mammalian body. Moreover, counterions such as acetate can protect compacted nucleic acid complexes from adverse effects of lyophilization

20020160516, Oct 31, 2002

Jan 14, 2002

10/043289

Mark Cooper - Moreland Hills OH, US

C12N015/86
C12N007/01

435/456000, 435/235100, 435/320100

A method is described for expressing biologically functional proteins without host cell toxicity. This method takes advantage of the surprising replication activating ability of the 107/402-T antigen. The invention also provides expression vectors, human cells, and fusion proteins for practicing the method. The invention thus provides the art with an expression system for proteins which is useful to the pharmaceutical and biotechnology industries

20030078229, Apr 24, 2003

Nov 27, 2002

10/305078

Mark Cooper - Moreland Hills OH, US
Murali Pasumarthy - Twinsburg OH, US
Tomasz Kowalczyk - University Heights OH, US
Maureen Costello - Beachwood OH, US

Copernicus Therapeutics, Inc. - Cleveland OH

A61K048/00
C12Q001/68
C07H021/04

514/044000, 435/006000, 536/023100, 514/002000

Counterions of polycations used to compact nucleic acids profoundly affect shape and stability of particles formed. Shape is associated with differential serum nuclease resistance and colloidal stability. A surrogate for determining such properties that is easy to measure is the turbidity parameter. Shape also affects the suitability and efficacy of compacted nucleic acid complexes for transfecting cells by various routes into a mammalian body. Moreover, counterions such as acetate can protect compacted nucleic acid complexes from adverse effects of lyophilization