Christopher R Lloyd

6750006, Jun 15, 2004

Jan 22, 2002

10/054419

Linda S. Powers - Logan UT
Christopher R. Lloyd - North Logan UT

Microbiosystems, Limited Partnership - Cheyenne WY

C12Q 100

435 4, 435 49

Method and apparatus for the detection of microbes in liquids, in air and on non-living surfaces in which samples are exposed to electromagnetic radiation of specific energies capable of exciting various metabolites, cofactors and cellular and spore components, with the microbial cells to be sampled (and more specifically the excited metabolites, cofactors and or other cellular components) contained therein emit fluorescence that can be measured. The signal from the background and scattered excitation signals is removed from the fluorescence signals of the microbial components, the relative fluorescent signals of the intrinsic microbial components are required to lie within physiological ranges, and the amplitude of the background-corrected fluorescence signals used to enumerate the microbe content in the sample.

6780602, Aug 24, 2004

Nov 1, 2001

09/999159

Linda S. Powers - Logan UT
Christopher R. Lloyd - North Logan UT

Microbiosystems, Limited Partnership - Cheyenne WY

G01N 3353

435 71, 435 4, 435 5, 435 72, 422 8205, 436164, 436172, 436536, 436538

The present invention describes a method for the binding of pathogenic microorganisms and their toxic proteins with ligands that have been covalently tethered at some distance from the surface of a substrate: distances of at least fifteen are required for microorganism binding ligand tethers and at least six are required for protein binding ligand tethers. The ligands described herein include heme compounds, siderophores, polysaccharides, and peptides specific for toxic proteins, outer membrane proteins and conjugated lipids. Non-binding components of the solution to be analyzed are separated from the bound fraction and binding is confirmed by detection of the analyte via microscopy, fluorescence, epifluorescence, luminescence, phosphorescence, radioactivity, or optical absorbance. By patterning numerous ligands in an array on a substrate surface it is possible to taxonomically identify the microorganism by analysis of the binding pattern of the sample to the array.

7186990, Mar 6, 2007

Apr 2, 2004

10/817647

Linda S. Powers - Logan UT, US
Christopher R. Lloyd - Logan UT, US

Microbiosystems, Limited Partnership - Cheyenne WY

G01N 21/64
C12Q 1/00

2504611, 2504581, 2504591, 435 4

Method and apparatus for the detection of biological material on non-living surfaces in which samples are exposed to electromagnetic radiation of specific energies capable of exciting various intrinsic fluorophores, and these fluorophores emit fluorescence that can be measured. The signal from the background, scattered excitation light and reflected excitation light is removed from the fluorescence signals due to the intrinsic fluorophores from the biological material and the intensities of the signals from the intrinsic fluorophores are required to lie within expected ranges.

7211377, May 1, 2007

Apr 2, 2004

10/817649

Linda S. Powers - Logan UT, US
Christopher R. Lloyd - Logan UT, US

Microbiosystems, Limited Partnership - Cheyenne WY

C12Q 1/00
C12P 35/06

435 4, 435 49

Method for the detection of dormant cryptobiotic microbes by detection of electromagnetic radiation emitted from intrinsic alkali earth metal pyridine dicarboxylic acid salts in the 710 nm to 860 nm region when excited with electromagnetic energy in the 610 nm to 680 nm region. Utilizing the novel lower energy emission of intrinsic calcium dipicolinic acid salts makes it possible to quickly detect bacterial spores, fungal spores and oocysts without the need for any added reagents, sample processing, or contact with the sample.

7824883, Nov 2, 2010

Jul 10, 2007

11/827226

Linda S. Powers - Logan UT, US
Christopher R. Lloyd - Logan UT, US

C12Q 1/04
C12Q 1/00
C12Q 1/06
C12Q 1/16

435 34, 435 4, 435 35, 435 39

Method and apparatus for the detection of microbes in liquids, in air and on non-living surfaces in which samples are exposed to frequency-modulated electromagnetic radiation of specific energies capable of exciting various metabolites, cofactors and cellular and spore components, with the microbial cells to be sampled (and more specifically the excited metabolites, cofactors and/or other cellular components) contained therein emit fluorescence that can be measured that is similarly frequency-modulated provided that the excitation frequencies are longer than the fluorescence lifetime of the excited intrinsic microbial fluorophore. The contribution to the measured signal due to each excitation frequency resulting from microbial intrinsic fluorescence is determined with a method capable of calculating the intensity of each frequency-modulated signal, and the relative total fluorescent signals of the intrinsic microbial components associated with each excitation frequency are required to lie within physiological ranges, with the amplitude of the fluorescence signals used to enumerate the microbe content in the sample.

20040096910, May 20, 2004

Nov 12, 2003

10/706547

Linda Powers - Logan UT, US
Walther Ellis - Logan UT, US
Christopher Lloyd - North Logan UT, US

G01N033/53

435/007100

The present invention describes a method for the binding of pathogenic microorganisms and their toxic proteins with ligands that have been covalently tethered at some distance from the surface of a substrate: distances of at least fifteen are required for microorganism binding ligand tethers and at least six are required for protein binding ligand tethers. The ligands described herein include heme compounds, siderophores, polysaccharides, and peptides specific for toxic proteins, outer membrane proteins and conjugated lipids. Non-binding components of the solution to be analyzed are separated from the bound fraction and binding is confirmed by detection of the analyte via microscopy, fluorescence, epifluorescence, luminescence, phosphorescence, radioactivity, or optical absorbance. By patterning numerous ligands in an array on a substrate surface it is possible to taxonomically identify the microorganism by analysis of the binding pattern of the sample to the array.

20040171137, Sep 2, 2004

Dec 31, 2003

10/749329

Linda Powers - Logan UT, US
Christopher Lloyd - North Logan UT, US

C12Q001/02
C12M001/34

435/283100, 435/029000, 435/287100

Method and apparatus for the detection of microbes in liquids, in air and on non-living surfaces in which samples are exposed to electromagnetic radiation of specific energies capable of exciting various metabolites, cofactors and cellular and spore components, with the microbial cells to be sampled (and more specifically the excited metabolites, cofactors and/or other cellular components) contained therein emit fluorescence that can be measured. The signal from the background and scattered excitation signals is removed from the fluorescence signals of the microbial components, the relative fluorescent signals of the intrinsic microbial components are required to lie within physiological ranges, and the amplitude of the background-corrected fluorescence signals used to enumerate the microbe content in the sample.

20050026140, Feb 3, 2005

Nov 12, 2003

10/706542

Linda Powers - Logan UT, US
Walther Ellis - Logan UT, US
Christopher Lloyd - North Logan UT, US

C12Q001/70
C12Q001/68
G01N033/53
G01N033/569
G01N033/554

435005000, 435006000, 435007310, 435007320

The present invention describes a method for the binding of pathogenic microorganisms and their toxic proteins with ligands that have been covalently tethered at some distance from the surface of a substrate: distances of at least fifteen Å are required for microorganism binding ligand tethers and at least six Å are required for protein binding ligand tethers. The ligands described herein include heme compounds, siderophores, polysaccharides, and peptides specific for toxic proteins, outer membrane proteins and conjugated lipids. Non-binding components of the solution to be analyzed are separated from the bound fraction and binding is confirmed by detection of the analyte via microscopy, fluorescence, epifluorescence, luminescence, phosphorescence, radioactivity, or optical absorbance. By patterning numerous ligands in an array on a substrate surface it is possible to taxonomically identify the microorganism by analysis of the binding pattern of the sample to the array.